GREEN SYNTHESIS OF SILVER NANOPARTICLES USING CHITOSAN HYDROLYSATE AS STABILIZING AGENT AND THEIR ANTIBACTERIAL ACTIVITY
Silver nanoparticles were successfully synthesized with chitosan hydrolysate as stabilizing agent at room temperature. Silver nitrate, glucose and sodium hydroxide were used as silver precursor, reducing agent and accelerator respectively. Chitosan hydrolysate was produced by enzymatic process with papain enzyme. Molecular weight of chitosan hydrolysate determined by viscosimetry methode based on Mark-Howink equation. The effect of molecular weight of chitosan hydrolysate as stabilizing agent and AgNO3 concentration toward surface plasmon resonance (SPR) of silver nanoparticle was investigated. It is also reported the antibacterial activity of silver-chitosan nanocomposites against Staphylococcus aureus and Escherichia coli. The products of silver nanoparticles were characterized by UV-Vis spectroscopy and transmission electron microscopy (TEM). The result showed that the formation of silver nanoparticles was shown by the appearance of surface plasmon resonance (SPR) at 403–421nm from the corresponding UV-Vis spectra. The molecular weight of chitosan hydrolysate affects the absorbance intensity and the wavelength of SPR. Silver nanoparticles were spherical in shape as identified by TEM images with size in range of 4–26nm. The silver nanoparticles have showed high antibacterial activity against Escherichia coli and Staphylococcus aureus.
Key word: green synthesis, silver nanoparticles, chitosan hydrolysate, stabilizing agent, antibacterial activity
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Chen JI., Han CM., Lin XW., Tang ZJ., Su SJ. 2006. Effect of silver nanoparticle dressing on second degree burn wound. Chinese journal of surgery. 44 (1):50-52.
Chudasama B., Vala AK., Andhariya N., Mehta RV. and Upadhyay RV. 2010. Highly bacterial resistant silver nanoparticles: synthesis and antibacterial activities. J. Nanopart. Res. 12:1677-1685.
Cai J., Kimura S., Wada M., Kuga S. 2009. Nanoporous cellulose as metal nanoparticles support. Biomacromolecules. 10:87–94.
Darroudi M., Ahmad MB., Abdullah AH., Ibrahim NA., Shameli K. 2010. Effect of Accelerator in Green Synthesis of Silver Nanoparticles. Int. J. Mol. Sci. 11:3898-3905.
Faraha AA., Alvarez-Puebla RA. Fenniri H. 2008. Chemically stable silver nanoparticle-crosslinked polymer microspheres. J. Colloid Interf. Sci., 319:572–576.
Fouda MMG. 2005. Use of Natural Polysacharides in Medical Textile Application. Disertasi. Fedbereich Chimie Universitat Duisburg-Essen. Germany.
García-Barrasa J., López-de-Luzuriaga JM., Monge M. 2011, Silver nanoparticles: synthesis through chemical methods in solution and biomedical applications. Cent. Eur. J. Chem. 9(1):7-19.
Goy RC., Britto DD., Assis OBG. 2009. A Review of the Antimicrobial Activity of Chitosan. Polímeros: Ciência e Tecnologia, 19(3):1-7.
Guzmán MG., Dille J., Godet S. 2009. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. I.J.Chem. Biological Engineering. 2(3):104-111.
Heath JR. 1989. Size dependent surface plasmon resonances in bare silver particles. Phys. Rev. B. 40:9982-9985.
Honary S., Ghajar K., Khazaeli P., Shalchian P. 2011. Preparation, Characterization and Antibacterial Properties of Silver-Chitosan Nanocomposites Using Different Molecular Weight Grades of Chitosan. Trop. J.Pharm. Res. 10(1):69-74.
Huang H., Yuan Q., Yang X. 2004. Preparation and characterization of metal-chitosan nanocomposites. Colloid Surface B. 39:31–37.
Kim,KM., Son JH., Kim SK., Weller CL., Hanna MA. 2006. Properties of Chitosan Films as a Function of pH and Solvent Type. J. Food Sci. E: Food Engineering and Physical Properties, 71(3):119–124.
Lin, H., Wang H., Xue C. and Ye M. 2002. Preparation of chitosan oligomers by immobilized papain. Enzyme and Microbial Technology. 31:588–592.
Luo C., Zhang Y., Zeng X., Zeng Y., Wang Y. 2005. The role of poly(ethylene glycol) in the formation of silver nanoparticles. J. Colloid Interf. Sci. 288:444–448.
Marambio-Jones C., Hoek EV. 2010. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J. Nanopart Res. 12:1531–1551.
Pranoto Y., Rakshit SK., Salokhe VM. 2005. Enhancing antimicrobial activity of chitosan ﬁlms by incorporating garlic oil. LWT-Food Sci Technol. 38 :859–65.
Qin, C., Du, Y., Xiao, L., Li Z. and Gao, X. 2002. Enzymic preparation of water-soluble chitosan and their antitumor activity. Int. J. Biol. Macromol. 31:111-117.
Raveendran P., Fu J., Wallen, S.L. 2003. Completely "green" synthesis and stabilization of metal nanoparticles. J. Am. Chem. Soc. 125:13940–13941.
Roe D., Karandikar B., Bonn-Savage N., Gibbins B., Roullet JB. 2008. Antimicrobial surface functionalization of plastic catheters by silver nanoparticles. J. Antimicrob. Chemother. 61 (4): 869-876.
Singh N., Sinha I., Mandal RK. 2009. Role of pH in the green synthesis of silver nanoparticles. Materials Letters. 63:425–427
Shameli K., Ahmad MB., Yunus WMZW., Ibrahim NA., Gharayebi Y., Sedaghat S., 2010, Synthesis of silver/ montmorillonite nanocomposite using γ-irradiation, Int. J. Nanomed., 5:1067–1077
Šileikaitė A., Puišo J., Prosyčevas I., Tamulevičius S. 2009. Investigation Of Silver Nanoparticles Formation Kinetic During Reduction Of Silver Nitrate With Sodium Citrate. Mater. Sci.(Medžiagotyra). 15(1):21-27
Vorobyova SA., Lesnikovich AI., Sobal NS. 1999. Preparation of Silver Nanoparticles by Interphase Reduction. Colloids Surf. A. 152:375 – 379
Xie H. 2011. Preparation of Low Molecular Weight Chitosan by Complex Enzymes Hydrolysis. Int. J.Chem.3(2):180-186
Zheng M., Gu M., Jin Y., Jin G. 2001. Optical Properties of Silver-Dispersed PVP Thin Film. Mater. Res. Bull. 36:853–859.
Zhou Y., Yu SH., Wang CY., Li XG., Zhu YR., Chen ZY. 1999, A novel ultraviolet irradiation photo-reduction technique for preparation of single crystal Ag nanorods and Ag dendrites. Adv. Mater. 11:850–852.
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