The effect of variation of dose, time toward cauli flower (Brassica oleracea var BotrytisL.) On hepatic cytochrome P-450 level rats given theophylline

Endang Sri Sunarsih, Lukman hakim, Sugiyanto ., Sumantri .

Abstract


Cytochrome  P-450  as  a  major  component  enzyme  system  in  drug metabolism. Activity  enzyme  of  cytochrome  P-450  was  influenced  by  internal and external factors. Vegetables of Brassicaceae such as cauli flower were often consumed  in  the  long  term,  its   has  inductor  activity  of  oxidation  enzyme systems and conjugation reactions. Theophylline as a bronchodilator drugs have the unknown effects on hepatic microsomal enzyme, such as cytochrome P-450 and   have  not  been  many  studies  that  tried  to  prove  it.  The  purpose  of  this study  was  to  prove  the  effects  of Cauliflower  and  indol  on  level  cytochrome  P-450  enzyme.  90  rats  were  divided  into  3  groups.  Group   I,  were  given Theophylline  20  mg/kg  BW.  In  group  II  30  rats  were  treated  with  indole  1,2; 2,4; 3,6 mg/kg BW, and group III 30 rats were treated with cauliflower extract respectively  doses  100,  200,  300  g/kg  BW.  Each  dose  was  given  on  10  rats, each group were divided 2 sub-groups were treated for 5, 10 days. On the last day  of  treatment  were  given  Theophylline  20  mg/kg  BW.   Cytochrome  P-450 enzyme  levels  were  determined  by  the  method  of  Omura  and  Sato(Snell,  and Mullock,1987). An induction cauliflower and indole did not increased levels of hepatic  cytochrome  P-450.  The  long  treatment  and  the   increased  of administered  dose  did  not  enhanced  the  levels  of  hepatic  cytochrome  P-450 enzyme. cauliflower and indole contained in vegetables when consumed together with theophylline drug, would not affect the metabolism of theophylline

Key words: Cauli flower, cytochrome P-450, theophylline, indole, spectrofotometric.


Full Text:

Untitled

References


Snell, K and Mullock, B, (editors) 1987, Biochemical toxicology, A Practical Approach IRL Press, Oxford Washington DC.

ndulski, JA and Lutz, W. 2000. Metabolic genotype in relation toindividual susceptibility to environmental carcinogenesis.Int Arch Occup Environ Health 73:71–85.

Akagah B, Lormier A.T, Fournet A, Figadere B, 2008., Oxidation of antiparasic 2- substituted quinolones using metalloporphyin catalysts : scale-up of a biomimetic reaction for metabolite production of drug candidates. Organic & Biomolecular Chemistry. 6. (24) : 4494-7.

Dalimartha, S. 2000. Atlas Tumbuhan Obat Indonesia.Jilid II. Jakarta : Trubus Agriwidya Lee S-A., Fowke J H., Lu W., Ye C., Zheng Y., Cai Q., Gu K., Gao y-T., Shu X-O., Zheng W; 2008; American Journal of Clinical Nutrition, March, vol. 87, No 3, 753-760.

Anonim, 1986, Sediaan Galenik, Departemen Kesehatan Republik Indonesia, Dirjen Pengawasan Obat Dan Makanan, Jakarta.

Anonim, (www.asiamaya.com, 2009).

Guengerich, F.P. 2005 Human cytochrome P450 enzymes. In Cytochrome P450: Structure Mechanism, and Biochemistry, 3rd ed.(Ortiz de Montellano, P. R., Ed.) pp 377–530, Kluwer Academic/Plenum Press, New York.

Oscarson M, Ingelman-Sundberg M, Daly AK, Nebert DW,2003.Human cytochrome P450 (CYP) alleles(web site) http://www.imm.ki.se/CYPalleles/

Nebert DW, & Russell DW. Clinical importance of the cytochromes P450. Lancet; 2002, 360:1155–1162.

Hallstrom H, Melhus, H, Glynn, A, Lind, L, Sylvanen, AC and Michaelsson, K, Coffee consumption and CYP1A2 genotype in relation to bone mineral density of the

proximal femur in elderly men and women : a cohort study, Nutrition & Metabolism, 2010, 7,(1) : 12.

Perera, V, CYP1A2*F is not a “rapid metabolizer” phenotype.Faculty of Pharmacy, The University of Sydney. (2011-06-21 17:55)

Murray,S. A.O.A,.Odupitan, B. P.,Murray, A.R.,Boobis and Edwards,R.J, 2001; Inhibition of human CYP1A2 activity in vitro by methylxanthines : potent competitive inhibition by 8-phenyltheophyllineVol. 31, No. 3, 135-151

Guengerich, F. P. 2001 Common and uncommon cytochrome P450 reactions related to metabolism and chemical toxicity. Chem. Res. Toxicol. 14, 611–650.

Louisa, M., Suyatna, F.D., Setiawati, A., Jusman, S.W.A., 2009., The Effect of Lycopene on total cytochrome P-450, CYP1A2 and CYP2E1, Med.J.Indones., Flockhart, (2007) : 233-8., vol.Flockhart, (2007), No.4, Oktober-Desember 2009.

Donatus, I.A., 1994. Antaraksi kurkumin dengan parasetamol. Kajian terhadap aspekfarmakologi dan toksikologi perubahan hayati parasetamol.Desertasi S3Universitas Gadjah Mada, Yogyakarta, Indonesia.

Gorski JC, Huang S-M, Pinto A, Hamman MA, Hilligoss JK, Zaheer NA, Desai M, Miller M and Hall SD. 2004. The effect of echinacea on cytochrome P-450 activity in vivo. Clin Pharmacol Ther, 75:89-100,

Flockhart DA. 2007. Drug Interactions: Cytochrome P450 Drug Interaction Table. Indiana University School of Medicine. http://medicine.iupui.edu/ clinpharm/ddis/ table. Asp. Accessed [date].




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp323-329

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

web
analytics View My Stats