Biosynthesis of a biopolymer poly(3-hydroxybutyrate) from a mixture of palm oil and 2-butanol as carbon sources

Akmal Djamaan

Abstract


Polyhydroxybutyrate  [P(3HB)]  are  synthesized  as  carbon  and  energy reserve materials by many types of microorganisms under certain environmental condition. These biosynthetic polyesters have much received attention as they can  be  considered  to  be  a  source  for  developing  novel  biodegradable  plastic materials for pharmaceutical, medical and biodegradable packages industries. In this case,  Erwiniasp. USMI-20, a locally soil isolated microorganism has been found  to accumulate  P(3HB)  in its  cells  during  growth  on  mineral-media  with  a mixture of palm oil and 2-butanol as carbon source. Fermentation process was conducted  through  a  feedbatch  cultivation  under  aerobic  condition  at  pH  7.0, incubation temperature 30 oC, and agitation rate of 200 rpm for 48 hours. The characterization of the  polymer production  was based  on  cell growth  (biomass) and  polymer  content  detected  by  a  gas  chromatography  methode.  Result showed  that  from  a  mixture  of palm  oil:2-butanol  (4.62g/L+0.89g/L) produced P(3HB) of 50.86 %w/w with biomass of 5.82g/L; a mixture of palm oil:2-butanol (4.62g/L+1.65g/L) produced  P(3HB)  of 57,77%  w/w  with  biomas  of 6.01g/L,  a mixture  of  palm  oil:2-butanol  (4.62g/L+2.48g/L)  produced  P(3HB)  of  55.85%

w/w with  biomass  of  6.57g/L,   a  mixture  of palm  oil:2-butanol (4.62g/L+3.29 g/L) produced P(3HB) of 56.37% w/w with biomass of 6.67g/L and a mixture of palm  oil:2-butanol  (4.62g/L+4.12g/L)  produced  P(3HB)  of  47.70%  w/w  with biomass of 6.33g/L

Key words:biopolymer, poly(3-hydroxybutyrate), palm oil, and n-butanol.


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References


Anderson, A. J. and Dawes, E. A. (1990), Occurrence, metabolism, metabolic role, and industrial uses of bacterial of polyhydroxy alkanoates, Microbiol. Rev.54: 450-472.

Braunegg, G., Sonnleitner, B. and Lafferty, R. M. (1978). A rapid gas chromatography method for the determination of poly--hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol. 6: 29-37.

Djamaan, A., M.N. Azizan and M.I.A. Majid , 2003. Biodegradation of Microbial Polyesters P(3HB) and P(3HB-co-3HV) under The Tropical Climate Environment, Int. J. Polym. Dedrad. Stab. 80: 513-518.

Djamaan, A. 2004. Penghasilan dan Pencirian P(3HB) dan P(3HB-ko-3HV) dari Berbagai Sumber Karbon oleh Erwinia sp USMI-20. Tesis Doktor Falsafah. Penang: Universitas Sains Malaysia.

Djamaan, A. 2010. Mikroorganisme dan Penggunaannya dalam Berbagai Bidang. Andalas University Press, Padang.

Djamaan, A. 2011. Konsep Produksi Biopolimer P(3HB) dan P(3HB-ko-3HV) Secara Fermentasi, Andalas University Press, Padang.

Doi, Y. 1990. Microbial Polyester, UCH Publ. Inc. New York.

Eggink, g., de Waard, P. and Huijberts, G. N. M. (1992). The role of fatty acid biosynthesis and degradation in the supply of substrates for poly(3-hydroxyalkanoate) formation in Pseudomonas putida. FEMS Microbiol. Rev.103: 159-164.

Fukui, T. and Doi, Y. (1998). Efficient production of polyhydroxyalkanoates from plant oil by Alcaligenes eutrophusand its recombinant strain. Appl. Microbiol. Biotechnol.49: 333-336.

Gonzalez-Garcia, Y., Nungaray J., Córdova J., González R., Koller M., Atlic M. & Braunegg G. 2008. Biosynthesis and Characterization of Polyhydroxyalkanoates in The Polysaccharide-degrading Marine Bacterium Saccharophagus degradans ATCC 43961, J. Indian Microbiol. Biotechnol., DOI 10.1007/s10295-007-0299-0

Lee, S. Y. (1996). Bacterial polyhydroxyalkanoates, Biotechnol. Bioeng. 49: 1-14.

Majid, M. I. A., Akmal, D., Few, L. L., Agustien, A., Toh, M. S., Samian, M. R., Najimudin, N. & Azizan, M. N. 1999. Production of Poly(3-hydroxybutyrate) and Its Copolymer Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Erwinia sp. USMI-20. Int. J. Biol. Macromol. 25: 95-104.

Majid, M.I.A., K. Hori., M. Aklyama & Y. Doi. 1994. Production of Poly(3-Hydroxybutyrate) from Plant Oil by Alcaligenes sp. Biodegradable Plastics and Polymers, Elsevier Science B. V, Amterdam.

Nubia M., Ivonne M., Dionisio M., Victoria G., Dolly R., Diego S., Juan G., Fabio A., Armando E and Dolly M. 2007. Bioprospecting and characterization of poly-hydroxyalkanoate (PHAs) producing bacteria isolated from Colombian sugarcane producing areas, African J. of Biotechnol., 6 (13)1536-1543.

Page, W.J. 1995. Bacterial Polyhydroxyalkanoates, Natural Biodegradable Plastic with a Great Future. Canadian J. Microbiol. 41(suppl.1): 1-3

Rezwan K., Q. Z. Chen., J. J. Blaker & A. R Boccaccini. 2006. Biodegradable and Bioactive Porous Polymer/Inorganic Composite Scaffolds for Bone Tissue Engineering. Biomaterials, 27: 3413-3431

Williams, S. F., Martin, D. P., Horowitz, D. M & Peoples, O. P. 1999. PHA Aplication: Addressing the Price Performance Issue I. Tissue Enginering. Int. J. Biol. Macromol.25: 111-121.




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp315-322

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