Biosynthesis of a biopolymer poly(3-hydroxybutyrate) from a mixture of palm oil and 2-butanol as carbon sources
Polyhydroxybutyrate [P(3HB)] are synthesized as carbon and energy reserve materials by many types of microorganisms under certain environmental condition. These biosynthetic polyesters have much received attention as they can be considered to be a source for developing novel biodegradable plastic materials for pharmaceutical, medical and biodegradable packages industries. In this case, Erwiniasp. USMI-20, a locally soil isolated microorganism has been found to accumulate P(3HB) in its cells during growth on mineral-media with a mixture of palm oil and 2-butanol as carbon source. Fermentation process was conducted through a feedbatch cultivation under aerobic condition at pH 7.0, incubation temperature 30 oC, and agitation rate of 200 rpm for 48 hours. The characterization of the polymer production was based on cell growth (biomass) and polymer content detected by a gas chromatography methode. Result showed that from a mixture of palm oil:2-butanol (4.62g/L+0.89g/L) produced P(3HB) of 50.86 %w/w with biomass of 5.82g/L; a mixture of palm oil:2-butanol (4.62g/L+1.65g/L) produced P(3HB) of 57,77% w/w with biomas of 6.01g/L, a mixture of palm oil:2-butanol (4.62g/L+2.48g/L) produced P(3HB) of 55.85%
w/w with biomass of 6.57g/L, a mixture of palm oil:2-butanol (4.62g/L+3.29 g/L) produced P(3HB) of 56.37% w/w with biomass of 6.67g/L and a mixture of palm oil:2-butanol (4.62g/L+4.12g/L) produced P(3HB) of 47.70% w/w with biomass of 6.33g/L
Key words:biopolymer, poly(3-hydroxybutyrate), palm oil, and n-butanol.
Anderson, A. J. and Dawes, E. A. (1990), Occurrence, metabolism, metabolic role, and industrial uses of bacterial of polyhydroxy alkanoates, Microbiol. Rev.54: 450-472.
Braunegg, G., Sonnleitner, B. and Lafferty, R. M. (1978). A rapid gas chromatography method for the determination of poly--hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol. 6: 29-37.
Djamaan, A., M.N. Azizan and M.I.A. Majid , 2003. Biodegradation of Microbial Polyesters P(3HB) and P(3HB-co-3HV) under The Tropical Climate Environment, Int. J. Polym. Dedrad. Stab. 80: 513-518.
Djamaan, A. 2004. Penghasilan dan Pencirian P(3HB) dan P(3HB-ko-3HV) dari Berbagai Sumber Karbon oleh Erwinia sp USMI-20. Tesis Doktor Falsafah. Penang: Universitas Sains Malaysia.
Djamaan, A. 2010. Mikroorganisme dan Penggunaannya dalam Berbagai Bidang. Andalas University Press, Padang.
Djamaan, A. 2011. Konsep Produksi Biopolimer P(3HB) dan P(3HB-ko-3HV) Secara Fermentasi, Andalas University Press, Padang.
Doi, Y. 1990. Microbial Polyester, UCH Publ. Inc. New York.
Eggink, g., de Waard, P. and Huijberts, G. N. M. (1992). The role of fatty acid biosynthesis and degradation in the supply of substrates for poly(3-hydroxyalkanoate) formation in Pseudomonas putida. FEMS Microbiol. Rev.103: 159-164.
Fukui, T. and Doi, Y. (1998). Efficient production of polyhydroxyalkanoates from plant oil by Alcaligenes eutrophusand its recombinant strain. Appl. Microbiol. Biotechnol.49: 333-336.
Gonzalez-Garcia, Y., Nungaray J., Córdova J., González R., Koller M., Atlic M. & Braunegg G. 2008. Biosynthesis and Characterization of Polyhydroxyalkanoates in The Polysaccharide-degrading Marine Bacterium Saccharophagus degradans ATCC 43961, J. Indian Microbiol. Biotechnol., DOI 10.1007/s10295-007-0299-0
Lee, S. Y. (1996). Bacterial polyhydroxyalkanoates, Biotechnol. Bioeng. 49: 1-14.
Majid, M. I. A., Akmal, D., Few, L. L., Agustien, A., Toh, M. S., Samian, M. R., Najimudin, N. & Azizan, M. N. 1999. Production of Poly(3-hydroxybutyrate) and Its Copolymer Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Erwinia sp. USMI-20. Int. J. Biol. Macromol. 25: 95-104.
Majid, M.I.A., K. Hori., M. Aklyama & Y. Doi. 1994. Production of Poly(3-Hydroxybutyrate) from Plant Oil by Alcaligenes sp. Biodegradable Plastics and Polymers, Elsevier Science B. V, Amterdam.
Nubia M., Ivonne M., Dionisio M., Victoria G., Dolly R., Diego S., Juan G., Fabio A., Armando E and Dolly M. 2007. Bioprospecting and characterization of poly-hydroxyalkanoate (PHAs) producing bacteria isolated from Colombian sugarcane producing areas, African J. of Biotechnol., 6 (13)1536-1543.
Page, W.J. 1995. Bacterial Polyhydroxyalkanoates, Natural Biodegradable Plastic with a Great Future. Canadian J. Microbiol. 41(suppl.1): 1-3
Rezwan K., Q. Z. Chen., J. J. Blaker & A. R Boccaccini. 2006. Biodegradable and Bioactive Porous Polymer/Inorganic Composite Scaffolds for Bone Tissue Engineering. Biomaterials, 27: 3413-3431
Williams, S. F., Martin, D. P., Horowitz, D. M & Peoples, O. P. 1999. PHA Aplication: Addressing the Price Performance Issue I. Tissue Enginering. Int. J. Biol. Macromol.25: 111-121.
- There are currently no refbacks.
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian J Pharm indexed by:
View My Stats