Antibacterial activity of ethanolic extract and fraction of leaves of singawalang (Petiveria alliaceae) against resistant bacteria
One of the plants that grow in Indonesia, and still does not use widely is singawalang (Petiveria alliaceae) from Phytolaceae family. Traditional herbal medicine preparations contained of Petiveria alliacea in the Caribbean, Latin America, West Africa and other regions for hundreds years has been used to treat pain, colds, inflammation, tumor, bacterial and fungal infection,
hyperlipidemia, diabetes and other diseases. This study aims to determine the antibacterial activity of singawalang growth in Indonesia against resistant bacteria to antibiotics. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration(MBC) were determined by microdilution broth method and type of antibacterial action were determined by observing the profile of bacterial growth curve. Ethanol extract of dried leaves showed activity against bacteria i.e. MRSA (Methicillin-Resistant Staphylococcus aureus), MRCNS (Methicillin-Resistant Coagulase-Negative Staphylococci), VRE (VancomycinResistant Enterococcus). Further studies conducted by reviewing the type of work by looking at the growth curve and the observed changes in cell walls by scanning electron microscopy (SEM) at MRCNS (Methicillin-Resistant Staphylococcus coagulase-Negative). The results showed that ethanol extracts and all fractions showed good antibacterial activity against resistant bacteria, are bacteriostatic hexane fraction until the concentration of 8 MIC and change the morphology of the cell wall of MRCNS.
Key words:Singawalang, Antibacteria, Broth Microdilution, bacterial growth curve, SEM
Amer, F.A., El-Behedy, E.M., and Mohtady, H.A., 2008. New Target for Antibacterial Agents. Biotechnology and molecular Biology Reviews; 3(3), 46-57
Benevides, P.J.C., Young, M.C.M., Giesbrecht, A.M., Roque, N.F., and Bolzani, V.S.,. , 2001, Antifungal polysulphides from Petiveria alliacea L. Phytochemistry 57; 743–747
Betriu C, Gomez M, Sanchez A, Cruceyra A, Romero J, and Picazo JJ . 1994. Antibiotic Resistance and Penicillin Tolerance in Clinical Isolates of Group B Streptococci. Antimicrobial Agents and Chemotherapy; 38(9):2183.
Departemen Kesehatan RI. Parameter Standar Umum Ekstrak Tumbuhan Obat. 2000. Direktorat Jenderal Pengawasan Obat dan Makanan Direktorat Pengawasan Obat Tradisional; Jakarta
Departemen Riset dan Teknologi. 2010. Agenda Riset Nas ional 2010-2014. Lampiran keputusan Menteri Riset dan Teknologi.
Fabricant DS, Farnsworth NR. 2001. The value of plants used in traditional medicine for drug discovery. Environmental Health Perspectives; Mar:109(Suppl 1), 69–75
Holetz, F.B, Pessini, G.L, Sanches, N.R, Cortez, D.A, Nakamura, C.V, and Filho, B.P., 2002, Screening of Some Plants Used in the Brazilian Folk Medicine forthe Treatment of Infectious Diseases, Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 97: 000-000
Miksusanti., Jennie, B. S. L., Panco, B. dan Trimulyadi, G., 2008, Kerusakan Dinding Sel Escherichia coli K1.1 oleh Minyak Atsiri Temu Kunci (Kaempferia pandurata), Berita Biologi 9(1):1-8
Kim, S., Kubec, R., Musah, R.A., 2006; Antibacterial and antifungal activity of sulfur-containing compounds from Petiveria alliaceaL. J Ethnopharm104: 188–192
NCCLS. 2003. Methods for Dillution Antimicrobial Susceptibility Test for Bacteria That Grow Aerobically; Approved Standard; Sixth Edition
Sambatakou, H., Giamarellos, B.E.J., Grecka, P., Chryssouli, Z., and Giamarellou, H., 1998. Invitro activity and killing effect of quinupristin/dalfopristin (RP59500) on nosocomial Staphylococcus aureus and interactions with rifampicin and ciprofloxacin against methicillin-resistant isolates. J Antimicrob Chemother; 41:349-55
Tropical Plant Database–Anamu (Petiveria alliacea), Rain tree. http://www.rain-tree.com/ anamu.htm, Accessed on 12 January 2010
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