Antibacterial activity of ethanolic extract and fraction of leaves of singawalang (Petiveria alliaceae) against resistant bacteria

Yani Mulyani, Elin Yulinah Sukandar, I Ketut Adnyana

Abstract


One  of the plants  that grow  in Indonesia,  and  still does  not  use widely  is singawalang  (Petiveria  alliaceae)  from  Phytolaceae  family.  Traditional   herbal medicine  preparations  contained  of  Petiveria  alliacea  in  the  Caribbean,  Latin America,  West  Africa  and  other  regions  for  hundreds  years  has  been  used  to treat  pain,  colds,  inflammation,  tumor,  bacterial  and  fungal   infection,

hyperlipidemia,  diabetes and  other  diseases.  This  study  aims to determine  the antibacterial  activity  of  singawalang  growth  in  Indonesia  against  resistant bacteria  to  antibiotics.  The  minimum  inhibitory  concentration   (MIC)  and minimum  bactericidal  concentration(MBC)  were  determined  by  microdilution broth method and type of antibacterial action were determined by observing the profile of bacterial growth curve. Ethanol extract of dried leaves showed activity against bacteria i.e. MRSA (Methicillin-Resistant Staphylococcus aureus), MRCNS (Methicillin-Resistant  Coagulase-Negative  Staphylococci),  VRE  (VancomycinResistant  Enterococcus).   Further  studies  conducted  by  reviewing  the  type  of work  by  looking  at the  growth curve  and  the observed changes  in cell walls by scanning  electron  microscopy  (SEM)  at  MRCNS  (Methicillin-Resistant Staphylococcus  coagulase-Negative).  The  results  showed  that  ethanol  extracts and  all  fractions  showed  good  antibacterial  activity  against  resistant  bacteria, are  bacteriostatic  hexane  fraction  until  the  concentration  of  8  MIC  and  change the morphology of the cell wall of MRCNS.

Key words:Singawalang, Antibacteria, Broth Microdilution, bacterial growth curve, SEM

 


Full Text:

Untitled

References


Amer, F.A., El-Behedy, E.M., and Mohtady, H.A., 2008. New Target for Antibacterial Agents. Biotechnology and molecular Biology Reviews; 3(3), 46-57

Benevides, P.J.C., Young, M.C.M., Giesbrecht, A.M., Roque, N.F., and Bolzani, V.S.,. , 2001, Antifungal polysulphides from Petiveria alliacea L. Phytochemistry 57; 743–747

Betriu C, Gomez M, Sanchez A, Cruceyra A, Romero J, and Picazo JJ . 1994. Antibiotic Resistance and Penicillin Tolerance in Clinical Isolates of Group B Streptococci. Antimicrobial Agents and Chemotherapy; 38(9):2183.

Departemen Kesehatan RI. Parameter Standar Umum Ekstrak Tumbuhan Obat. 2000. Direktorat Jenderal Pengawasan Obat dan Makanan Direktorat Pengawasan Obat Tradisional; Jakarta

Departemen Riset dan Teknologi. 2010. Agenda Riset Nas ional 2010-2014. Lampiran keputusan Menteri Riset dan Teknologi.

Fabricant DS, Farnsworth NR. 2001. The value of plants used in traditional medicine for drug discovery. Environmental Health Perspectives; Mar:109(Suppl 1), 69–75

Holetz, F.B, Pessini, G.L, Sanches, N.R, Cortez, D.A, Nakamura, C.V, and Filho, B.P., 2002, Screening of Some Plants Used in the Brazilian Folk Medicine forthe Treatment of Infectious Diseases, Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 97: 000-000

Miksusanti., Jennie, B. S. L., Panco, B. dan Trimulyadi, G., 2008, Kerusakan Dinding Sel Escherichia coli K1.1 oleh Minyak Atsiri Temu Kunci (Kaempferia pandurata), Berita Biologi 9(1):1-8

Kim, S., Kubec, R., Musah, R.A., 2006; Antibacterial and antifungal activity of sulfur-containing compounds from Petiveria alliaceaL. J Ethnopharm104: 188–192

NCCLS. 2003. Methods for Dillution Antimicrobial Susceptibility Test for Bacteria That Grow Aerobically; Approved Standard; Sixth Edition

Sambatakou, H., Giamarellos, B.E.J., Grecka, P., Chryssouli, Z., and Giamarellou, H., 1998. Invitro activity and killing effect of quinupristin/dalfopristin (RP59500) on nosocomial Staphylococcus aureus and interactions with rifampicin and ciprofloxacin against methicillin-resistant isolates. J Antimicrob Chemother; 41:349-55

Tropical Plant Database–Anamu (Petiveria alliacea), Rain tree. http://www.rain-tree.com/ anamu.htm, Accessed on 12 January 2010




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp293-29

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

                                    
 
web
analytics View My Stats