Cytotoxicity and antimicrobial test of the bioactive compound isolated from Stylissa flabelliformis sponge

Erna Prawita Setyowati, Sudarsono ., Subagus Wahyuono

Abstract


A research of cytotoxicity and antimicrobial test of bioactive compound of Stylissa flabelliformis sponge have been done. Cytotoxicity test were conducted on myeloma cells, while antimicrobial test were conducted against Staphylococus aureus,Eschericia coli and Candida albican. The research was initiated with the isolation of bioactive compound from the active fraction of sponge S. Stylissa monitored by Brine shrimp Lethality tes (BST). The active fraction was chromatograped with silica gel as its immobile phase and the mixture of hexane and ethyl acetate (1:1 ) as the mobile phase to obtain compound 1,2,3 and 4.
Compound 3 has the most toxic character because it could kill A. salina equal to 100% at dose 25 mg/ml, and has LC50 of 0,9 mg/ml. Cytotoxicity test with direct staining method using tripan blue on myeloma cell at density of 4,5x10 cell / 100.ul showed that the compound 3 had a high activity against myeloma cell, having LC50 equal to 0,08 mg/ml.
The result of antimicrobial effect showed that compound 3 was a fungicide against Candida albicans but having no activity on Staphylococcus aureus and Eschericia coli
Key words: sponge, Stylissa flabelliformis , cytotoxicity, fungicide

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References


Hooper, J.N.A., 1997, Sponge Guide: Guide to Sponge Collection and Identification, pp : 33-38, version March, Quensland Museum, Brisbane.

Hooper, J.N.A and van Soest, R.W.M., 2002, Systema Porifera : A Guide to Classification of Sponges, pp : 783-784, volume 1, Kluwer Academic, Plenum Publisher, New York.

Setyowati, E.P., Sudarsono dan Wahyuono, S., 2003, Penentuan Fraksi Aktif Spons Stylissa flabellirormis yang toksik terhadap Artemia salina Leach, J.Teknosains, Seri A, IPA Jilid 16, nomor 3, hal 499-513 , Pascasarjana UGM, Yogyakarta




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp50-56

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