ISOLATION OF SULPHATE POLYGALACTAN FROM Eucheuma alvarezii Doty AND ITS ANTICOAGULANT ACTIVITY

Sutrisno ., Parlan ., Hanifah Nur Al-Fath

Abstract


A sulphate polygalactan carrageenan was isolated from Euchema alvarezii Doty with yield 54.36%. The sulphate polygalactan was fractionated with KCl (aq) 2.5% result of a sulphate polygalactan of soluble fraction with the yield 60.34% and a sulphate polygalactanof insoluble fraction with the yield 34.53%. Based on the properties and supported by IR spectrum, the sulphate polygalactan of soluble fraction is k-carrageenan, whilethe sulphate polygalactan of insoluble fraction is l-carrageenan. Anticoagulant activitiy of the carrageenans were based on their prolongation effects on Activated Partial Thromboplastine Time (APTT) and Prothrombine Time (PT) of sodium citrate. On the assays, the carrageenans exhibited promising anticoagulant activities.


Key words: Eucheumaalvarezii Doty, k-carrageenan, l-carrageenan, anticoagulant activity, APTT and PT


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References


Bois Boisson-Vidal C., Chaubet F., Chevolot L., Sinquin C., Theveniaux J. and Millet J. 2000. Relationship between anti thrombotic activities of fucans and their structure. Drug Development Research, 51, 216–224.

Farias WRL., Valente AP., Pereira MS. and Mourao PAS. 2000. Structure and anticoagulant activity of galactan: isolation of a unique sulfated galactan from the red algae Batryocladia occidentalis and comparison of its anticoagulant action with that of sulfated galactan from invertebrates. J. Biol. Chem. 275, 29299 – 29307.

Fonseca RJC., Oliveira SNMCG., Melo FR., Pereira MG., Benevides NMB. And Mourao PAS. 2008. Slight differences in sulfatation of algal galactanas account for differences in their anticoagulant and venous antithrombotic activities. Thromb. Haemost. 99, 539 – 545.

Ghos P., Adhikari U., Ghossal PK CA., Pujol MJ., Carlucci EB., Damonic and Ray B. 2004. In-vitro anti-herpatic activity of sulfated polysaccharide fraction from Caulerpa racemosa. Phytochem. 65,31513157.

Jiao G., Yu G., Zhang J. and Ewart HS. 2011. Chemicals structures and bioactivities of sulfated polysacharides from marine algae. Mar. Drugs. 9, 196 – 223.

Mao WJ., Fang F., Li HY., Qi XH., Sun HH., Chen Y. and Guo SD. 2008. Heparinoidactive two sulfated poly-saccharides isolated from marine green algae Monostroma nitidum. Carbohydr. Polym. 74, 834 – 839.

Matsubara K., Matsuura Y., Hori K. and Miyazawa K. 2000. An anticoagulantprotoglycan from marine green algae, Codiumpugniformis. J. Appl. Phycol.,12, 9-14.

Matsubara K., Matsuura Y., Bacic A., Long Liou M., Hori K. and Miyazawa K. 2001. Anticoagulant Properties of Sulfate Galactan Preparation from a Marine Green Algae, Codiumcylindricum. Int.. J. Biol.Macromol., 28, 395-399.

Mourao, P.A.S. 2004. Use of sulfated fucan as anticoagulant and antithrombotic agents: future perspectives. Curr. Pharm. Des. 10, 967 – 981.

Pereira MS., Melo FR. and Mourao PAS. 2002. Is there a correlation between structure and anticoagulant action of sulfated galactans and and sulfated fucan? Glycobiol. 12, 573 – 580.

Rodrigues JAG., Vanderlei ESO., Bessa EF., Megalhaes FA., M de Paula RC., Lima V. and Benevides NMB. 2011. Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides. Braz. Arch. Biol. Technol. 54 n 4, 691 – 700.

Shanmugam S. and Mody KH. 2000.

Heparinoid active sulphated polysachary- des from marine algae as potential blood anticoagulant agents. Curr. Sci.79, 16721683.

Sutrisno, Parlan, Sari IP. and Sari K. 2010. Aktivitas antikoagulan karagenan hasil isolasi dari Eucheuma spinosum. p KIM15–KIM-24. In Proceedings of Seminar Nasional MIPA-Peran MIPA dalam Pengembangan Teknologi dan Pendidikan Berkarakter menuju Bangsa Mandiri: 13

November 2010. FMIPA UM. Malang. Indonesia.

Zhang HJ., Mao WJ., Fang F., Li HY., Sun HH., Chen Y. and Qi XH. 2008. Chemical characteristics and anticoagulant activities of a sulfated polysaccharide and its fragments from Monostroma latissimun. Carbohydr. Polym. 71, 428 – 434.




DOI: http://dx.doi.org/10.14499/indonesianjpharm24iss2pp86-92

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