STANDARDIZATION OF CELERY LEAVES PREPARATION (Apium graveolens L.) BY THIN LAYER CHROMATOGRAPHIC – DENSITOMETRY USING APIGENIN AS PARAMETER

Djatmiko M., Pramono S.

Abstract


In order to apply a standardization method on herbal medicines or pharmaceuticals, a thin layer chromatographic-densitometry was used to determine apigenin as the active constituent of celery extract formulated in a capsule dosage form. Silica gel GF 254 and chloroform – methanol – water (70 – 30 – 6,5 v/v) were used as stationary and mobile phases, respectively. The areas of apigenin were evaluated at 340 mm using a TLC-Scanner.

The limit of detetion was 3 g and the accuracy was 89,6% after first extraction prosedure applied on celery capsules. The apigenin content was 0,017 %.

Key Words : Apium graveolens L., apigenin, TLC – densitometry.


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References


Anonim, 2000, Parameter Standar Umum Ekstrak Tumbuhan Obat, Dep.Kes. RI, Jakarta, 37-38.

Duke, 2001, Phytochemical Database, Beltswille Agricultural Research Centre, Beltswille, Maryland, (http://www.ars-grin.gov/cgi-in/duke/pharmacy-scroll-3.pl).

Perry L.M, 1980, Medicinal Plants of East and Southeast Asia, The Mitch Press, Massachusetts, 413.

Pramono S, Bayumurti Y, Santoso D, 2000, Penetapan Bilangan Parameter Standarisasi Mutu Ekstrak Simplisia Cabe Jawa, Laporan Uji Laboratorium, Fakultas Farmasi UGM, Yogyakarta, 24-29. Djatmiko M.

Mabry T.J., Markham K.R., Thomas M. B., 1970, The Systematic Identification of Flavonoids, Springer Verly; Berlin, 18-25.

Masruhen, 2000, Perbandingan Kadar Flavonoid dan Daya Antihipertensi Sari etanol 50 % Daun dan Biji Seledri (Apium graveolus L.) Terhadap Tekanan Darah Sistemik Kucing Dianestesi, Skripsi, Fakultas Farmasi UGM, Yogyakarta, 10 - 11.




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp59-64

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