Ability of fermented soymilk byLactobacillus plantarum 1 in bile acids binding
Abstract
Some lactic acid bacteria (LAB) strains have been isolated from spontaneously fermented soymilk which showing proteolytic activity. These isolated were expected could producing bioactive peptides that could lowering cholesterol content when they used for soymilk fermentation. This research was aimed to determine the proteolytic activity of Lactobacillus plantarum 1 R.1.3.2 and L. plantarum 1 R.11.1.2 comparing with L. acidophilus FNCC 0051 as a
control. Furthermore soymilk fermented with these isolates were monitored for their ability to bind bile acid and these results were compared with the control of soymilk with no fermentation. Proteolytic activities (U/mL) were measured spectrophotometrically using tyrosin as a standard product, while bile acid binding capacity (µM/100 mg protein) using Total bile Acid Binding Kit. The results show that all isolates use in this study had no different proteolytic activity nor bile acid binding capability to that of control strain. The fermentation process could increased their ability to bind with the bile acid.
Key words :fermented soymilk, proteolytic lactic acid bacteria, bile acid binding
Full Text:
PDF 202-208References
Bordignon, J. R., Nakahara, K., Yoshihashi T. and Nikkuni S. 2004. Hydrolysis of isoflavone and consumption of oligosacharides during lactic acid fermentation of soybean milk. JARQ 38 (4) 259- 265.
Erdmann, K., Cheung, B. W. Y. and Schröder. H.2008.The possible roles of food-derived bioactive peptides in reducing the risk of cardiovascular diseases. Journal of Nutritional Biochemistry. 19 : 643 – 654.
Garabal, J. I., Alonso, P. R. and. Centeno, J. A. 2007. Characterization of lactic acid bacteria isolated from raw cow’s milk cheeses currently produced in Galicia ( NW Spain). Swiss Soc. of Food Sci. And Technol.
Gobbetti, M., Smacchi, E., Fox, P., Stepaniak, L. and Corsetti, A. 1996. The Sourdough Microflora. Cellular Localization and Characterization of Proteolytic Enzymes in Lactic Acid Bacteria. Academic Press Limited.
Kwon, D. Y., Oh, S. W., Lee, J. S., Yang, H. J., Lee S. H. and Lee, J. H. 2002. Amino acid substitution of hypocholesterolemic peptide originated from glycinin hydrolyzate, Food Science and Biotechnology11, pp. 55–61.
Lehninger, A. L. 1982. Principles of biochemistry.Worth Publisher, Inc.
Nakazawa Y. and Hosono, A. 1992. Function of fermented milk. Challenges for the health sciences. 180-184S
Nisa, F. Z., Marsono, Y .and Harmayani, E. 2006. Efek hipokolesterolemik susu kedelai fermentasi steril pada model hewan coba. Agrosains, 19(1); 41-53
SKRT. 2002. Perlu program penanggulangan penyakit kardiovaskular. SKRT :http//www.kompas.com/kompas-cetak/0206/iptek/per119.htm
Sugano, M. and Goto, S. 1990. Steroid binding peptides from dietary protein. J. of Nutrition Science and Vitaminology, 36 : s147 – s150
Walter, H. E. 1984. Method with Haemoglobin, Casein and Azocoll as Subtrate In. Bergmeyer, H.U. (ed). Methods of Enzymatic Analysis. Verlag Chemie. Deerfield Beach Florida Basel.
Yumiko, Y. S. and Wäsche, A. 2004. In vitro bindingof bile acids by lupin protein isolates and their hydrolysates. Food Chemistry88: 179 – 184.
Yusmarini, Adnan, M. and Hadiwiyoto, S. 2001. Perubahan nilai cerna dan fraksi protein pada susu kedelai dalam proses pembuatan soygurt. Agritech Vol. 21 No.3. 95-98.
Yusmarini, Indrati, R. Utami, T. and Marsono, Y. 2009. Isolasi dan identifikasi bakteri asam laktat proteolitik dari susu kedelai yang terfermentasi spontan. Jurnal Natur Indonesia 12.1
Zhong, F., Zhang, X., Ma, J. and Shoemake, C. F. 2007. Fractionation and identification of a novel hypocholesterolemic peptide derived from soy protein Alcalase hydrolysates. Food Research International, 40 756-762.
DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp202-208
Refbacks
- There are currently no refbacks.
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian J Pharm indexed by:
View My Stats