Ability of fermented soymilk byLactobacillus plantarum 1 in bile acids binding

Yusmarini ., Indrati R., Utami T., Marsono Y


Some  lactic  acid  bacteria  (LAB)  strains  have  been  isolated  from spontaneously  fermented  soymilk  which  showing  proteolytic  activity.  These isolated  were  expected  could  producing  bioactive  peptides  that  could  lowering cholesterol content when they used for soymilk fermentation. This research was aimed to  determine  the  proteolytic activity of Lactobacillus  plantarum  1 R.1.3.2 and  L.  plantarum  1 R.11.1.2  comparing  with  L.  acidophilus FNCC  0051  as  a

control. Furthermore soymilk fermented with these  isolates were monitored for their ability to bind bile acid and these results were compared with the control of soymilk  with  no  fermentation.  Proteolytic  activities  (U/mL)  were  measured spectrophotometrically  using  tyrosin  as  a  standard  product,  while  bile  acid binding   capacity  (µM/100  mg  protein)  using  Total  bile  Acid  Binding  Kit.  The results  show  that  all  isolates  use  in  this  study  had  no  different  proteolytic activity nor bile acid binding capability to that of control strain. The fermentation process could increased their ability to bind with the bile acid.

Key words :fermented soymilk, proteolytic lactic acid bacteria, bile acid binding

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Bordignon, J. R., Nakahara, K., Yoshihashi T. and Nikkuni S. 2004. Hydrolysis of isoflavone and consumption of oligosacharides during lactic acid fermentation of soybean milk. JARQ 38 (4) 259- 265.

Erdmann, K., Cheung, B. W. Y. and Schröder. H.2008.The possible roles of food-derived bioactive peptides in reducing the risk of cardiovascular diseases. Journal of Nutritional Biochemistry. 19 : 643 – 654.

Garabal, J. I., Alonso, P. R. and. Centeno, J. A. 2007. Characterization of lactic acid bacteria isolated from raw cow’s milk cheeses currently produced in Galicia ( NW Spain). Swiss Soc. of Food Sci. And Technol.

Gobbetti, M., Smacchi, E., Fox, P., Stepaniak, L. and Corsetti, A. 1996. The Sourdough Microflora. Cellular Localization and Characterization of Proteolytic Enzymes in Lactic Acid Bacteria. Academic Press Limited.

Kwon, D. Y., Oh, S. W., Lee, J. S., Yang, H. J., Lee S. H. and Lee, J. H. 2002. Amino acid substitution of hypocholesterolemic peptide originated from glycinin hydrolyzate, Food Science and Biotechnology11, pp. 55–61.

Lehninger, A. L. 1982. Principles of biochemistry.Worth Publisher, Inc.

Nakazawa Y. and Hosono, A. 1992. Function of fermented milk. Challenges for the health sciences. 180-184S

Nisa, F. Z., Marsono, Y .and Harmayani, E. 2006. Efek hipokolesterolemik susu kedelai fermentasi steril pada model hewan coba. Agrosains, 19(1); 41-53

SKRT. 2002. Perlu program penanggulangan penyakit kardiovaskular. SKRT :http//www.kompas.com/kompas-cetak/0206/iptek/per119.htm

Sugano, M. and Goto, S. 1990. Steroid binding peptides from dietary protein. J. of Nutrition Science and Vitaminology, 36 : s147 – s150

Walter, H. E. 1984. Method with Haemoglobin, Casein and Azocoll as Subtrate In. Bergmeyer, H.U. (ed). Methods of Enzymatic Analysis. Verlag Chemie. Deerfield Beach Florida Basel.

Yumiko, Y. S. and Wäsche, A. 2004. In vitro bindingof bile acids by lupin protein isolates and their hydrolysates. Food Chemistry88: 179 – 184.

Yusmarini, Adnan, M. and Hadiwiyoto, S. 2001. Perubahan nilai cerna dan fraksi protein pada susu kedelai dalam proses pembuatan soygurt. Agritech Vol. 21 No.3. 95-98.

Yusmarini, Indrati, R. Utami, T. and Marsono, Y. 2009. Isolasi dan identifikasi bakteri asam laktat proteolitik dari susu kedelai yang terfermentasi spontan. Jurnal Natur Indonesia 12.1

Zhong, F., Zhang, X., Ma, J. and Shoemake, C. F. 2007. Fractionation and identification of a novel hypocholesterolemic peptide derived from soy protein Alcalase hydrolysates. Food Research International, 40 756-762.

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp202-208


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