Suppression of DMBA-induced carcinogenesis of breast cancer in post initition stage by ethanolic extract of Gynura procumbens (Lour), Merr leaves

Edy Meiyanto, Sri Tasminatun, Sri Susilowati, Retno Murwanti, Sugiyanto .

Abstract


Gynura procumbens (Lour) Merr., has been shown to suppress lung cancer development in mice and breast cancer development in rat when the extract was given at initiation stage. The aim of this research is to examine the potential of ethanolic extract of G. procumbens to suppress DMBAinduced breast cancer development at early development (post initiation I) and late development (post initiation II). Sprague Dawley Rats were used in this research and were grouped as indicated treatment. Ethanolic extract of G. procumbens was administered into 2 levels of doses, namely 250, 750 mg/kgBW. Tumor development was examined by palpation every week and terminated at week 16th after the end of DMBA treatment. The result showed that extract treatment at the dose of 250, and 750 mg/kgBW at the post initiation I could not reduce tumor incidence but suppressed of tumor multiplicity. However, the treatment at the post initiation II, the extract could not reduce neither incidence nor multiplicity. In conclusion, ethanolic extract of G. procumbens performs potential effect to suppress breast cancer development at the dose of 250 mg/kgBW when administered at the early stage of carcinogenesis.

Key words : Carcinogenesis inhibition, G. procumbens, breast cancer, post initiation


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References


Cory S., and Adams M., 2002, The BCl-2 Family: Regulators of the Cellular Life or Death Switch, Nature Rev., 2, 647-656

Hanahan, D., and Weinberg, R.A., 2000, The Hallmarks of Cancer, Cell 100, 57-70

Jenie, R. I., Meiyanto, E., and Murwanti, R, 2006, Efek antiangiogenik ekstrak etanolik daun sambung nyawa (Gynura procumbens (Lour.) Merr.) pada membran korio alantois (cam) embrio ayam, Majalah Farmasi Indonesia, 17, 50 –55.

Jenie, R. I., and Meiyanto, E., 2007, Aplikasi ko-kemoterapi ekstrak etanolik daun Gynura procumbens (Lour.) Merr. pada sel kanker payudara, Majalah Farmasi Indonesia, 18, 81-87.

Kerbel R., and Folkman J., 2002, Clinical Translation of Angiogenesis inhibitor, Nature Rev., 2, 727 - 739.

Kubatka, P., Ahlersova E., Ahlers I., Bojkova B., Kalicka K., Adamekova E., Markova M., Chamilova, M., and Cermakova, M., 2002, Variability of Mammary Carsinogenesis Induction in Female Sprague-Dawley and Wistar : Han Rats : the Effect of Season and Age, Physiol. Res. , 51, 633 - 640.

Meiyanto, E., Susilowati, S., Tasminatun, S., Murwanti, R., and Sugiyanto, 2007, Efek kemopreventif ekstrak etanolik Gynura procumbens (Lour), Merr pada karsinogenesis kanker payudara tikus, MFI, 18, 154 – 161.

Murkies, A. L., Wilcox, G., and Davis, S. R., 1998, Phytoestrogens, J Clin Endocrinol Metab 83, 297 - 303.

Nooble MEM., Endicott JA., and Johnson LN., 2004, Protein Kinase Inhibitors: Insights into Drug Design from Structure (Rev.), Science, 303, 1800 - 1805

Rana P.Singh1, Puja Agrawal1, Dongsool Yim1, 2,Chapla Agarwal1,3 and Rajesh Agarwal, 2005, Acacetin inhibits cell growth and cell cycle progression, and induces apoptosis in human prostate cancer cells: structure-activity relationship with linarin and linarin acetate, Carcinogenesis , 26, 845 - 85.

Singletary, K., MacDonald, Iovinelli, M., Fisher, C., and Walling, M., 1998, Effect of the betadiketones diferuloylmethane (curcumin) and dibenzoylmethane on Rat Mammary DNA adducts and Tumor induced by 7,12-dimethylbenz(a)anthrazene, Carcinogenesis, 19, 1039 - 1043

Sugiyanto, Sudarto, B., Meiyanto, E., Nugroho, A. E., and Jenie U. A., 2003, Aktivitas antikarsinogenik Senyawa Yang Berasal Dari Tumbuhan, Majalah Farmasi Indonesia, 14, 132 - 141

Zhai. S., Dai, R., Friedman,F., and Vestal,R., 1998, Comparative Inhibition Of Human cytochromes P450 1A1 and 1A2 By Flavonoids, Drug Metabolism and Disposition, 26, 989 - 992




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp169-175

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