Chemopreventive effect of ethanolic extract of Gynura procumbens (Lour), Merr on the carcinogenesis of Rat breast cancer development

Edy Meiyanto, Sri Susilowati, Sri Tasminatun, Retno Murwanti, Sugiyanto .


Gynura procumbens (Lour) Merr., empirically, used to prevent cancer development and has been proven to be able to suppress lung cancer development. The aim of this research is to examine the potential of ethanolic extract of G. procumbens to suppress DMBA-induced breast cancer development. Sprague Dawly Rats were used in this research and were grouped as indicated treatment. Ethanolic extract of G. procumbens was administered into 3 levels of doses, namely 250, 500, and 750 mg/kgBW. Tumor development was examined by palpation every week and terminated at week 16th after the end of DMBA treatment. The result showed that extract treatment at the dose of 250, 500, and 750 mg/kgBW reduced tumor incidence by 60%, 30 %, and 20 % respectively. The doses of 500 and 750 mg/kgBW exhibited strong suppression of tumor multiplicity, where as the dose of 250 performed less potential suppression. In conclusion, ethanolic extract of G. procumbens performs chemopreventive effect to suppress breast cancer development at the dose of 250 mg/kgBW.

Key words : chemopreventive, Gynura procumbens (Lour) Merr, breast cancer.

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Dandekar, S., Sukumar S., Zaebl H., Young L. J. T., and Cardiff R. D., 1986, Specific activation of the cellular Harvey-ras oncogene in DMBA-induced Mouse mammary tumors, Mol. Cell Biol., 6, 11, 4104-4108

Dean, M., 1998, Cancer as a Complex Developmental Disorder, Cancer Res 58, 5633-5636

Hanahan, D., and Weinberg, R. A., 2000, The Hallmarks of Cancer, Cell 100, 57-70

Kubatka, P., Ahlersova E., Ahlers I., Bojkova B., Kalicka K., Adamekova E., Markova M., Chamilova M., and Cermakova M., 2002, Variability of Mammary Carsinogenesis Induction in Female Sprague-Dawley and Wistar : Han Rats : the Effect of Season and Age, Physiol. Res. , 51, 633-640

Limtrakul, P., Anuchapreeda S., Lipigorngoson S., and Dunn F. W., 2001, Inhibition of carcinogen induced c-Ha-ras and c-fos protooncogenes expression by dietary curcumin, BMC Cancer, 1 (1).

Meiyanto, E., and Septisetyani, E. P, 2005, Efek antiproliferatif dan apoptosis fraksi fenolik ekstrak etanolik daun Gynura procumbens terhadap sel HeLa, Artocarpus, (5) 2, 74-80

Ngyen, T., Sherratt P. J., and Pickett C. B., 2003, Regulatory mechanisms controlling gene expression mediated by the antioxydant response element, Annu Rev Pharmacol Toxicol, 43, 233-260

Rundle, A., Tang D., Hibshoosh H., Estabrook A., Schanabel F., Cao W., Grumet S., and Perera F., 2000, The relationship between genetic damage from polycyclic aromatic hydrocarbons in breast tissue and breast cancer, Carcinogenesis, 2 (7), 1281-1289

Singletary, K., MacDonald, Iovinelli, M., Fisher, C., and Walling, M., 1998, Effect of the betadiketones diferuloylmethane (curcumin) and dibenzoylmethane on Rat Mammary DNA adducts and Tumor induced by 7,12-dimethylbenz(a)anthrazene, Carcinogenesis, 19, 6, 1039-1043

Singletary, K., Macdonald, C., and Wallig, M., 1997, The Plasticizer Benzyl Butyl Phtalate (BBP) Inhibits 7,12-dimethylbenz(α)anthracene (DMBA)-induced rat Mammary DNA Adduct Formation and Tumorigenesis, Carsinogenesis , 18, 8, 1669-1673

Sugiyanto, Sudarto, B., dan Edy Meiyanto, A. E Nugraha, and U. A Jenie., 2003, Efek Penghambatan Karsinogenisitas Benzo(a)piren Oleh Preparat Tradisional Tanaman Gynura sp. Dan Identifikasi Awal Senyawa Yang Berkhasiat, MFI, 14, 3, 132-141

Zhai, S., Dai, R., Friedman,F., and Vestal,R., 1998, Comparative Inhibition Of Human Cytochromes P450 1A1 and 1A2 By Flavonoids, Drug Metabolism and Disposition, 26, 10, 989-9.



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