Co-chemotherapy of sambung nyawa (Gynura procumbens (Lour.) Merr.) leaves ethanolic extract and Doxorubicin on breast cancer cell

Edy Meiyanto; Riris Istighfari Jenie

doi:10.14499/indonesianjpharm0iss0pp81-87

Co-chemotherapy of sambung nyawa (Gynura procumbens (Lour.) Merr.) leaves ethanolic extract and Doxorubicin on breast cancer cell

Edy Meiyanto, Riris Istighfari Jenie

Abstract

Chemotherapy combination attracted high attention in recent years to cure cancer. This method combines between non-toxic or less toxic phytochemicals and chemotherapeutic agents to sensitize cancer cell, to enhance the efficacy of chemotherapeutic agents as well as to reduce its toxicity to normal tissues. The aim of the present research was to examine whether ethanolic extract of Sambung Nyawa leaves (SNE) or Gynura procumbens (Lour.) Merr. synergizes the therapeutic potential of doxorubicin (Dox) on breast cancer cell line T47D.

MTT assay was used to measure the effect of growth inhibitory of the combination therapy on T47D cells. SNE (25-500 μg/mL) treatment of cell resulted in 12-97.% growth inhibition in a dose dependent manner (IC50 90 μg/mL), while Dox (1.8-90 nM) treatment showed inhibitory effect of 16-83.% (IC50 50 nM). The combinations of SNE with Dox (1.8-18 nM) showed synergistic effect (CI<1). These results demonstrate a strong possibility of synergistic efficacy of SNE and Dox combination for breast cancer treatment.

Key words: Sambung Nyawa leaves ethanolic extract, doxorubicin, cochemotherapy, T47D human mammary cancer cell.

Full Text:

Untitled

References

Davis, J.M., Navolanic, P.M., Weinstein-Oppenheimer, C.R., Steelman, L.S., Wei H., Konopleva, M., Blagosklonny, M.V., and McCubrey, J.A., 2003, Raf-1 and Bcl-2 Induce Distinct and Common Pathways That Contribute to Breast Cancer Drug Resistance, Clinical Cancer Research, Vol. 9, 1161-1170.

Kitagawa, S., 2006, Inhibitory Effect of Polyphenols on P-Glycoprotein-Mediated Transport, Biol. Pharm. Bull., 29(1), 1-6.

Meiyanto, E., dan Septisetyani, E.P., 2005, Efek Antiproliferatif dan Apoptosis Fraksi Etanolik Ekstrak Etanolik Daun Gynura procumbens (Lour.) Merr. Terhadap Sel HeLa, Artocarpus, Vol.5, No.2, hal 74-80.

Notarbartolo, M., Poma, P., Perri, D., Dusonchet, L., Cervello, M., and Alessandro, N., 2005, Antitumor effects of curcumin, alone or in combination with cisplatin or doxorubicin, on human hepatic cancer cells. Analysis of their possible relationship to changes in NF-кB activation levels and in IAP gene expression, Cancer Letter, 224, 53-65.

Reynolds, C.P., and Maurer, B.J., 2005, Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models, Methods Mol. Med., 110: 173-183.

Sharma, G., Tyagi, A.K., Singh, R.P., Chan, D.C.F., and Agarwal, R., 2004, Synergistic anti-cancer effect of grape seed extract and conventional cytotoxic agent doxorubicin against human breast carcinoma cells, Breast Cancer Research and Treatment, 85:1-12.

Singal, P.K., and Iliskovic, N., 1998, Doxorubicin-induced cardiomyopathy, N Engl J Med., 339:900-905.

Sugiyanto, Sudarto, B., Meiyanto, E., Nugroho, A.E., dan Jenie, U.A., 2003, Aktivitas antikarsinogenik senyawa yang berasal dari tumbuhan, Majalah Farmasi Indonesia, 14(4):216-225.

Tyagi, A.K., Agarwal, C., Chan, D.C.F., and Agarwal, R., 2004, Synergistic anti-cancer effects of silibinin with conventional cytotoxic agents doxorubicin, cisplatin and carboplatin against human breast carcinoma MCF-7 and MDA-MB468 cells, Oncology Reports, 11:493-499.

Wattanapitayakul, S.K., Chularojmontri, L., Herunsalee, A., Charuchongkolwongse, S., Niumsakul, S., and Bauer, J.A., 2005, Screening of Antioxidants from Medicinal Plants for Cardioprotective Effect against Doxorubicin Toxicity, Basic & Clinical Pharmacology & Toxicology, vol.96, 80.

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp81-87