Isolation and structure elucidation of main compound originated from sponge Axynissa aplysinoides

Wahono Sumaryono, Agung Eru Wibowo, Chaidir .

Abstract


Isolation of the main compound of sponge Axynissa aplysinoides collected from Lombok seawater, has been conducted. Methanol extract of dried sponge was fractionated into nonpolar, semipolar and polar fractions using hexane, ethyl acetate and buthanol. Identification and final purification of the main compound was done using column chromatography and high performance liquid chromatography (HPLC). Structure elucidation of the main compound was performed using mass spectrometry (EIMS), 1H- and 13C-NMR spectroscopies. The main compound was identified as (E)-(4-hydroxystyryl) trimethylammonium.
Key words : isolation, structure elucidation, Axynissa aplysinoides

Full Text:

Untitled

References


Abraham R.J, Fisher J and Loftus P, 1988, Introduction to NMR Spectroscopy, John Wiley & Sons Chichester- New York-Brisbane-Toronto-Singapore

Dudley, H.W and Fleming, I, 1995, Spectroscopy methods in organic chemistry, fifth edition, Toronto

Edrada, R.A, 1998, Isolation and Structure Elucidation of Bioactive Compound Secondary Metabolites from Philippine Marine Sponges and Soft Coral, Dissertation, Departement of Pharmaceutical Biology, Wuerzburg University, Germany

Heller, U., 1999, Isolation, Biological Activity and Secondary Metabolite Investigation of Marinedeivaed Fungi and Selected Host Sponges, Dissertation, Gemeinsamen Naturwissenschaftichen Fakultat der Technischen Universitat Carolo-Wilhelmina,Braunschweig, Germany

Higa, T., Tanaka, J.I., and Tan, L.T., 1998, Cytotoxic macrocycles from marine sponges dalam Attaur-Rahman ang M. Iqbal Coudhary, New Trends in Natural Product Chemistry, Harwood Academic Publisher

Hooper, J.N.A, 2000, Sponguide : Guide to sponge collection and identification, Queensland Museum, South Brisbane, Autralia, http://www.qmuseum.qld.au/naturewel-come

Reinaldo S.C and D. John Faulkner, 1995, Metabolites of the Palauan Sponge Axynissa aplysinoides, Journal of Natural Products, Vol. 58, No. 1, pp 145-148.

Van Soest, R.W.M, 1994, “Demosponge distribution pattern, In : Van Soest, R.W.M, Van Kompen, T.M.G, Braekman, J.C. (Eds), Sponge in Time and Space”, A.A. Balkema, Rotterdam




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp186-191

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

web
analytics View My Stats