Determination of antioxidant activity of dehydrozingerone through hydroxy radical scavengers using deoxyribosa method
Dehydrozingerone (4-(4-hydroxy-3-methoxyphenyl)-3-buten-2-one) is an analog of curcumin which is termed as half-curcumin. The compound is isolated from ginger (Zingiber officinale). Nevertheless, this compound still has a phenolic hydroxy moiety responsible for antioxidant activity. The research was performed to obtain information on the antioxidant activity of dehydrozingerone through hydroxy radical scavengers.
The study was performed according to a hydroxy radical scavenging procedur. In this method, reaction between hydroxy radical, resulted from phenton reaction, and deoxyribosa produces malonedealdehyde. Incubation in thiobarbituric acid at low pH produced violet-coloured solution (complex MDA-TBA).
The results showed that dehydrozingerone has antioxidant activity through hydroxy radical scavengers with ES15 value of 48.36 μM. The antioxidant activity of dehydrozingerone was lower than curcumin, with ES15 value of 25.01 μM. Besides, exchange of phenolic hydroxy moiety by salting with potassium could deplete its antioxidant activity. Based on these facts, it can be concluded that phenolic hydroxy moiety of dehydrozingerone was important for its antioxidant activity through hydroxy radical scavengers.
Key words : dehydrozingerone, curcumin, antioxidant, radical hydroxy, Zingiber
Aggarwal, B.B., Kumar, A., Aggarwal, M.S., and Shishodia, S., 2003, Curcumin Derived From Turmeric (Curcuma longa): A Spice for All Seasons, in Phytochemicals in Cancer Chemoprevention, CRC Press LLC, p. 1-24.
Araújo, C.A.C and Leon, L.L., 2001, Biological Activities Curcuma longa L., Mem Inst. Oswaldo Cruz, 96(5) : 723-728.
Kunchandy, E., and Rao, M.N.A., 1990, Oxygen Radical Scavenging activity of Curcumin, Inter. J.. Pharm., 58, 237-240.
Kuo, P.C., Damu1, A.G., Cherng, C.Y, Jeng, F., Teng, C.M, Lee, E.J, and Wu,T.S., 2005, Isolation of a Natural Antioxidant, Dehydrozingerone from Zingiber officinale and Synthesis of Its Analogues for Recognition of Effective Antioxidant and Antityrosinase Agents, Arch Pharm Res, 28(5), 518-528.
Majeed, M., Badmaev, V., Shivakumar, U., and Rajendran, R., 1995, Curcuminoids: Antioxydant Phytonutrients, Nutri Science Publisher Inc., Piscataway, New Jersey, p. 32-63.
Masuda, T., Hidaka, K., Shinohara, A., Maekawa, T., Takeda, Y. and Yamaguchi, H., 1999, Chemical Studies on Antioxidant Mechanism of Curcuminoid : Analysis of Radical Reaction Products from Curcumin, J. Agric. Food Chem., 47, 71-77.
Motohashi, N, Ashihara, Y, Yamagami, C, and Saito, Y., 1996, Antimutagenic effects of ehydrozingerone and its analogs on UV-induced mutagenesis in Escherichia coli, Mutation Research, 377, 17–25.
Motohashi, N., Yamagamia, C., Tokudab, H., Konoshimac, T., Okudab, Y., Okudab, M., Mukainakab, T., Nishinob, H., and Saitod, Y., 1998, Inhibitory effects of induced Epstein-Barr virus early antigen activation, Cancer Letters, 134 : 37-42.
Nugroho, A.E., Supardjan, A.M., Hakim, L., Istyastono, E.P., dan Yuniarti, N., 2004, Sintesis dan Uji Aktivitas Biologis Senyawa-Senyawa Baru Turunan 1,5-Bis(4’-Hidroksi-3’- Metoksifenil)-1,4-Pentadien-3-On, Laporan Penelitian Hibah Bersaing XII, Universitas Gadjah Mada.
Sun, You-Min, Zhang, Hong-Yu, Chen, De-Chan and Liu, Cheng-Bu, 2002, Theoritical Elucidation on the Antioxidant Mechanism of Curcumin : A DFT Study, Shandong University, Jinan.
Tonnesen, H. H. and Greenhill, J.V., 1992, Studies on Curcumin and Curcuminoid XXII : Curcumin as a Reducing Agent and as a Radical Scavenger, Int. J. Pharm., 87, 79-87.
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