Toxicity effect of Centella asiatica Linn. extract on mice (Mus musculus) organ and tissue
Toxicity test ofCentella asiaticaLinn. water extract had been carried out. Extract were administered via oral, single and daily doses in mice (Mus musculus). The effect of extract on organs and tissue were evaluated by observing the pathology anatomy of heart, liver and kidney and also
LD50 value. The influences of acute and sub-chronic toxicity on morpho-anatomy organs and histo-pathology tissue were in accordance with LD50experiment. The results of morpho-anatomy showed that all organs were still in normal condition at low dose treatment, but stain and white spots appeared on liver, black spot on heart and white spots on kidney at higher dose. Histo-pathology indicated that low dose administration of extract resulted that, all of tissue observed were in good and normal condition, but at higher dose resulted injure of muscle fibres and also injure of nucleus cells of heart tissue also the colour of liver tissue was brick red and white stain manifestation (because the damaged of cells and accumulation of leucocytes). Degeneration and congestive sinus occurred in liver tissue, while necrosis and protein degeneration formed in kidney because of glomerulus membrane damaged. LD50 of Centella extract was 13.6 g/kg. This dose indicated that Centella extract is slightly toxic (5 - 15 g/kg), so that it is suggested that if it was used in phyto-therapy had to be under supervision of an expert or specialist and administered in low dose and short period.Keywords : Centella asiatica Linn; extract; acute and subchronic toxicity; anatomy pathology
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Chaudory S, Ghosh S, Chakraborty T, Kundu S, and Hazra S. K., 1978. Use of a Common Indian Herb “Mandukaparni” in The Treatment of Leprosy, J. Indian M.A70: 77-80.
Dash B. 1995. Aryurvedic Cures for Common Diseases, Hind Pocket Book (P) Ltd.; 107. Huang, Y. H., Zhang S. H., Zhen R. X., Xu X. D., and Zhen Y. S. 2004. Asiaticosida inducing apoptosis of tumor cells and enhancing anti-tumor activity of vincristine. Ai Zheng 23, 12, 1599-1604.
Loedin, A. A., 1999, Peran riset dalam Pendayagunaan Potensi Obat Tradisional Sebagai Unsur dalam Pelayanan Kesehatan Masyarakat, Seminar sehari Pendayagunaan Potensi Obat Tradisional Sebagai Unsur Dalam Pelayanan Kesehatan Masyarakat, BPPT, 9 Maret 1999.
Loomis T. A., 1975. Acute and Prolong Toxicity Test, J. Assoc. Off. Analytical Chem.58; 645.
Ratnaningsih A., 2000. Pengaruh Kadmium Terhadap Gangguan Potologik pada hati dan ginjal hewan percobaan, Thesis IPB; 27-49.
Sjamsoe-Daili E. S., 2002. Pengobatan Ulkus Kusta dengan Obat Tradisional, Berita IPTEK43, 1, LIPI; 57-64.
Tang, W and Eisenbrand, G. 1992. Chinese Drugs of Plants Origins; Chemistry, pharmacology and use in traditional and modern medicine, Springer-Verlag, Berlin; 273-276.
Turner R. A., 1965. Screening Methods in Pharmacology, Academic Press, New York and London; 61-63.
Winarto W. P, and Surbakti M., 2003. Khasiat dan Manfaat Pegagan, Agromedia Pustaka, 6-10.
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