Production of artemisinin in shoot cultures of Artemisia cinairradiated callus

Aryanti .

Abstract


Artemisinin  is  an  antimalaria  agent  that  is  only  found  in Artemisia  sp. medicinal  plant.  This  compound  is  occurred  at  very  low  level  in  that  plant. Irradiated callus of Artemisia cinahave been conducted by the dose of 40 Gy to improve  artemisinin content  in shoot  cultures.  Regeneration  of irradiated  callus to  shoot  cultures  formation  has  been  successful  in  Murashige  &  Skoog  (MS) medium  combined  with  Naphtalene  Acetic  Acid  (NAA)  1  mg/g,  Benzyl  Amino Purin (BAP) 2 mg/L and 15 % coconut water. Ten mutant lines of shoot cultures have  been  analyzed  for  the  artemisinin  content.  The  artemisinin  content  of mutant lines were higher than the original plant. The highest artemisinin content is  5  mg/g  found  in  mutant  line  number  404,  this  line  also  has  wider  leaf  area than original plant.

Key word : artemisinin, Artemisia cina, shoot cultures, irradiated callus


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References


Aryanti, Bintang, M., Ermayanti, T. M., and Mariska, I., 2001, Production of antileukemic agent in untransformed and transformed root culture of Artemisia cina. A J. of Biotechnology and Related Fundamental Sciences., 8, 1, 11 – 16.

Aryanti, Ermayanti, T. M, Prinadi, I. K, dan Dewi, R. M., 2006, Uji daya antimalaria Artemisia spp. Terhadap Plasmodium falciparum. Majalah Farmasi Indonesia, 17,2, 81 – 84.

Croteau, R., and McCaskill, D.,1994, Some caveats for bioengineering terpenoid metabolism in plants. TIBTECT16, 349 – 355.

Ferreira, J. F. S., and Janicks, J.,1996, Roots as enhancing factor for the production of artemisinin in shoot cultures of Artemisia annua. Plant Tissue and Organ Culture44, 211 – 217.

Geldre, E. V., Vergauewe, A., and Eeckhout E. V., 1997, State of the art the production of the antimalarial compound artemisinin in plants. Plant Molecular Biology, 33, 199 – 219.

Kelsey, R. G., and F Shafizadeh. 1980. Glandular trichomes and sesquiterpene lactones of Artemisisa nova. Biochem.Syst Ecol. 8, 317 – 377.

Nin. S., Moorosi, Schiff, E. ., and Bennici, A., 1996, Callus cultures of Artemisia absinthium L : initiation, growth optimization and organogenesis. Plant Cell, Tissue and Organ Culture 45, 67 – 72.

Woerdenbag, H., Luers, J. F. J., Uden van W., Pras, N., Malingre, T. T., and Alfermann A. W., 1993, Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua. Plant Cell, Tissue and Organ Culture. 32, 47 – 257.




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp27-31

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