Production of artemisinin in shoot cultures of Artemisia cinairradiated callus
Artemisinin is an antimalaria agent that is only found in Artemisia sp. medicinal plant. This compound is occurred at very low level in that plant. Irradiated callus of Artemisia cinahave been conducted by the dose of 40 Gy to improve artemisinin content in shoot cultures. Regeneration of irradiated callus to shoot cultures formation has been successful in Murashige & Skoog (MS) medium combined with Naphtalene Acetic Acid (NAA) 1 mg/g, Benzyl Amino Purin (BAP) 2 mg/L and 15 % coconut water. Ten mutant lines of shoot cultures have been analyzed for the artemisinin content. The artemisinin content of mutant lines were higher than the original plant. The highest artemisinin content is 5 mg/g found in mutant line number 404, this line also has wider leaf area than original plant.
Key word : artemisinin, Artemisia cina, shoot cultures, irradiated callus
Full Text:PDF 27-31
Aryanti, Bintang, M., Ermayanti, T. M., and Mariska, I., 2001, Production of antileukemic agent in untransformed and transformed root culture of Artemisia cina. A J. of Biotechnology and Related Fundamental Sciences., 8, 1, 11 – 16.
Aryanti, Ermayanti, T. M, Prinadi, I. K, dan Dewi, R. M., 2006, Uji daya antimalaria Artemisia spp. Terhadap Plasmodium falciparum. Majalah Farmasi Indonesia, 17,2, 81 – 84.
Croteau, R., and McCaskill, D.,1994, Some caveats for bioengineering terpenoid metabolism in plants. TIBTECT16, 349 – 355.
Ferreira, J. F. S., and Janicks, J.,1996, Roots as enhancing factor for the production of artemisinin in shoot cultures of Artemisia annua. Plant Tissue and Organ Culture44, 211 – 217.
Geldre, E. V., Vergauewe, A., and Eeckhout E. V., 1997, State of the art the production of the antimalarial compound artemisinin in plants. Plant Molecular Biology, 33, 199 – 219.
Kelsey, R. G., and F Shafizadeh. 1980. Glandular trichomes and sesquiterpene lactones of Artemisisa nova. Biochem.Syst Ecol. 8, 317 – 377.
Nin. S., Moorosi, Schiff, E. ., and Bennici, A., 1996, Callus cultures of Artemisia absinthium L : initiation, growth optimization and organogenesis. Plant Cell, Tissue and Organ Culture 45, 67 – 72.
Woerdenbag, H., Luers, J. F. J., Uden van W., Pras, N., Malingre, T. T., and Alfermann A. W., 1993, Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua. Plant Cell, Tissue and Organ Culture. 32, 47 – 257.
- There are currently no refbacks.
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian J Pharm indexed by:View My Stats