Analysis of compounds possessing inhibitory properties on mice locomotor activity from essential oils of Ki Lemo bark (Litsea cubeba lour. Pers)
Inhalation of essential oil of ki lemo bark (Litsea cubeba Lour. Pers) at the doses of 0.5 ml inhibit locomotor up to 60,75 %, whereas at the doses of 0.1 ml and 0.3 ml inhibit locomotor activity of 57,44 % and 54,20 %, respectively.
Identification and quantification of volatile active compounds in the blood plasma were carried out by GC-MS analysis after half an hour, one hour, and two hours inhalation. The blood plasma of three mice were collected in heparin tube, isolated and concentrated by the C-18 column (100 mg-Seppak Waters) eluted with methanol and bidistilled water (60:40).
The major compounds identified were citronellol, citronellal, α-terpineol, and 1,8-cineole. The blood level of those substances was increasing for one hour after inhalation but decreasing after two hours.
Key words : essential oil of ki lemo bark (Litsea cubeba Lour. Pers), inhalation, locomotors.
Chen, W.Y., Ko, F.N., Wu, Y.C., Lu, S.T., and Teng, C.M. 1994. Vasorelaxaing effect in rat thoracic aorta caused by lauratanine isolated from Litsea cubeba Persoon. J. Pharm. and Pharmacol.; 46(5): 380-382.
Choi, Eun-Mi and Jae-Kwan Hang. 2004. Effect of methanolic extract and fraction from Litsea cubeba bark on the production of inflammatory mediators in RAW264.7 cells. J. Fitoterapia.; 75: 141-148.
Jirovetz, L., Buchbauer, G., Jager W., Woidich, A., and Nikiforov, A.. 1992. Analysis of fragrance coumpound in blood samples of mice by Gas Chromatography, Mass Spectrometry, GC/FTIR, & GC/AES after inhalation of s&alwood oil. J. Bio. Chrrom., 6, 133-134.
Kovar, K. A., B. Grooper, D. Fries and H. P. T. Ammon. 1987. Blood levels of 1,8-cineole & locomotor acivity of mice after inhalation & oral administration of rosemary oil. J.Planta Medicinal; 53: 315-318.
Mardisiswojo S. and Radjakmangunsudarso. 1968. Cabe puyang warisan nenek moyang. Karya Wreda : Jakarta. hal 636
Rugayah., Sulistiarini D., Djarwaning T., and Widjaya E.A. 1989. South east Asian spices: present state and futeture as exemplified by Indonesian cooking. Proceeding of the fist PROSEA Intern. Siemonsma J.S. & Wulijarni, Editors. May 22-25 1989; 154-163.
Susiarti S. 1996. Peran Balemg la (Litsea cubeba) sebagai tumbuhan obat dan aroma pada masyarakat Dayak kenyah di Pujungan Kalimantan Timur. Prosiding Simposium Nasional Tumbuhan Obat dan Aromatik APINMAP. 6-7 Oktober 1996; 634-639.
Wang, F., Yang, D., Ren, S., Zhang, H., and Li, R. 1999. Chemical compotition of essential oil frm leaves of Litsea cubeba and its antifungal activities. Zhong Yao Cai.; 22(8): 400-402. (Abstract: Agricola).
Wu, C.Y., Jin-Yuan, L., Chang-Yih, D., Shoei-Sheng Lee, and Sheng-Teh Lu. 1991. Litebamene , a novel phenantrene alkaloid from Litsea cubeba. Tetrahedron letters; 32 (33): 4169-4170.
- There are currently no refbacks.
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian J Pharm indexed by:
View My Stats