Snake beans (Vigna sinensis (L) Savi ex Hassk) extract increases breast epithelial cells proliferation

Edy Meiyanto, Sri Handayani, Riris Istighfari Jenie

Abstract


Some Javaness people use snake beans for skin and breast care, especially for better breast development. This research was conducted to examine the effect of snake beans extract (EKP) on the breast epithelial cells proliferation on in vitro and in vivo models. The in vitro experiment was carried out against MCF-7 cells using MTT assay and morphologically examination was carried out under light microscope. Sprague Dawley female Rats were used in in vivo experiment. The rats (30 days of age) were separated into 3 groups, namely base line group, control group, and treatment groups. The extract was administered in the dose of 1000 mg/kgBW p.o. every day for 14 days then the rats were examined for wet uterus weight, lobulus development, and estrogen receptor (ER) expression. Extract treatment induced MCF-7 cells proliferation in dose dependent manner. The extract exhibited proliferative effect in the dose of 50 ug/mL 300 ug/mL, but in the dose of 400 ug/mL and 500 ug/mL the extract inhibited cells proliferation and there were no cell death effect. Extract treatment in the dose of 1000 mg/kgBW tended to increase uterus weight. The extract also increased lobulus development up to two fold and induced estrogen receptor expression in epithelial cells of lobulus and ductus. These results conclude, snake bean (in appropriate dose) induces breast glands development and relatively safe (no death effect on the cells), therefore can be developed for breast care product.

Key words: Snake bean, breast care, proliferation, lobulus epithelial cells, estrogen receptor


Full Text:

pdf 191-197

References


Benassayag, C., Perrot-Applanat, M. and Ferre, F., 2002, Phyroestrogens as Modulators of Steroid

Action in Target Cells, J. Chrom. B, 777 : 233 – 248.

Foster, J. S., Henley, D.C., Ahamed, S., Wimalasena, J., 2001, Estrogen and Cell Cycle Regulation in Breast Cancer, Trend in Endocrinology and Metabolism, 12 (7), 320-327.

Ji, S., Willis G.M., Frank G.R. Cornelius S.G., and Spurlock M.E., 1999, Soybean Isoflavones, Yenistein and Yenistin In hibit Rat Myoblast Proliferation, Fusion and Myotube Protein Sinthesis, J. Nutr, 129 : 1291 – 1297.

Leeson, C. R., Leeson, T. S. and Paparo, A. A., 1989, Textbook of Histology, WB Saunders Company, Philadelpia.

Messina, M. J. and Loprinzi, C. L., 2001, Soy for Breast Cancer Survivors: A Critical Review of the Literature, J. Nutr., 131: 3095S–3108S.

Murkies, A. L., Wilcox, G., Davis, S. R., 1998, Phytoestrogens, J Clin Endocrinol Metab 83:297-303.

Nilsson, S., Makela, S., Treuter, E., Tujague, M., Thomsen, J., Andersson, G., Enmark, E., Petterson K., Warner, M., Gustafsson, J., 2001, Mechanisms of Estrogen Action, Physiol Rev., Vol 81, 1536-1565.

Santell, R. C. Chang, Y. C., Nair M. G. and Helferich W. G., 1997, Dietary Genistein Exerts Estrogenic Effec Upon the Uterus, Mammary Gland and the Hypotalamic/Putuitary Axis in Rats, J. Nutr, 127: 263 - 269

Watanabe, H., Suzuki, A., Goto, M., Lubahn, D. B., Handa, H., Iguchi, T., 2004, Tissue-specific estrogenic and non-estrogenic effects of a xenoestrogen, nonylphenol, Journal of Molecular Endocrinology, 33, 243–252.

Wong, Y. S., and Chang, Q., 2004, Identification of flavonoids in Hakmeitau beans (Vigna sinensis) by high-performance liquid chromatography-electrospray mass spectrometry (LCESI/ MS), J. Agric. Food Chem., 52: 22, 6694 - 6699

Xu, S. Y., Zhu, B. T., Cai, M. X., Conney, A. H., 2001, Stimulatory Effect of Clofibrate on the Action of Estradiol in the Mammary Gland but Not in the Uterus of Rats, JPET, 297:50–56.

Zampieri L, Bianchi P, Ruff P, Arbuthnot P, 2002, Differential modulation by estradiol of Pglycoprotein drug resistance protein expression in cultured MCF7 and T47D breast cancer cells, Anticancer Res., 22(4):2253-9.




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp191-197

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

                                    
 
web
analytics View My Stats