Molecular identification and anticancer activity of alkylphenol from cashew nut shell oil (Anacardium occidentale) grown In Timor Island
Isolation of anacardic acid and cardanol from cashew nut shell liquid and their cytotoxic activity to on ward HeLa cancer cell line has been done. The objective of this research is to isolate and identify anacardic acid and cardanol from CNSL along with their cytototoxic activity towards HeLa cancer cell lines. Anacardic acid was isolated as calcium anacardate. Hydrolisis of this acid with acid chloride yields anacardic acid . Liquor ammonia was added to the acid-free CNSL and extracted with hexane/ethyl acetate (98:2) to isolate cardanol and with ethyl acetate/hexane (80:20) to separate cardol. Cardanol was obtained as the main chemical component of CNSL derived from Kupang district. Anacardic acid and cardanol was analyzed using IR and GC-MS. Anticancer activity of chemical components of CNSL against HeLa cancer cell lines were studied using MTT. Cytotoxic activity test showed that CNSL, anacardic acid and cardanol can be developed as anticancer agent.
Key words : CNSL, anacardic acid, cardanol, anticancer.
Balasubramanyam K., Swaminathan V., Ranganathan A., and Kundu T. K.. 2003. Small Moleculer Modulators of Histone Acetyltransferase p300. J. Bio. Chem. 278, 19134-19140
Begum Parvin et al, 2002. Zoosporocidal Activites of Anarcadic Acid against Aphanomyces cochliodes. Z. Naturforsch 57c, 874-882
Itokawa H et al, 1987. Antitumor Principle from Ginkgo Biloba L. Chem. Pharm. Bull. 35, 3016-3020
Kozubek A, Zarnowski R, Stasiuk M and Gubernator J, 2001. Natural Amphiphilic phenols as bioactive compounds. Cellular & Molecular Biology Letters 6:351-355
Kumar PP, Paramashivappa R, Vithayathil PJ and Rao AS. 2001. Process for Isolation of of Cardanol from Technical Cashew (Anacardium Occidentale L.) Nut Shell Liquid. J. Agric Food Chem. 50: 4705-4708
Lee Ji S, Cho Y S, Park E J, Kim J, Oh W K, Lee H S and Ahn Jo S.1998. Phospholipase Cç1 Inhibitory Principles from the Sarcotestas of Ginkgo biloba. J. Nat. Prod., 61, 867-871
Paramashivappa R, Kumar PP, Vithayathil PJ and Rao AS. 2001. Novel Method for Isolation of Major Phenolic Constituens from Cashew (Anacardium Occidentale L.) Nut Shell Liquid. J. Agric Food Chem. 49 (5): 2548-2551
Risfaheri. 2005. Kajian Pemanfaatan Kardanol dari Cairan Kulit Biji Mete Sebagai Substitusi Fenol Dalam Formulasi Perekat Fenol Formaldehida. Disertasi, Sekolah Pasca Sarjana IPB.
Santos dos L.M. and Magalhaes de C.G. 1999. Utilization of Cashew Nut Shell Liquid from Anarcadium occidentale as Starting Material for Organic Synthesis: A Novel Route to Lasiodipladin from Cardols. J.Braz. Chem. Soc. 10: 13-20
Silverstein R M and Webster F X. 1997. Spectrometric Identification of Organic Compounds. John Wiley & Sons Inc, New York.
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