DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR DETERMINATION AND QUANTIFICATION OF RUBRAXANTHONE IN STEM BARK EXTRACT OF MANGOSTEEN

Meri Susanti, Dwisesaria Irma Lena, Dachriyanus Dachriyanus

Abstract


Rubraxanthone is one of antibacterial  and antioxidant compound which is isolated from stem bark of mangosteen (Garcinia mangostana Linn.). The precise method for analysis of plant constituents is normally a reverse-phase high performance liquid chromatographic (RP-HPLC). Development and validation of a RP-HPLC method for chromatographic separation was carried out on a reversed-phase column Shimadzu ®  Shimp-pack VP – ODS (4.6x250mm) using an isocratic mobile phase of  0.4% formic acid - acetonitrile at  a flow rate 1mL min-1, and detection was  with a UV detector. The linearity of  the proposed method was found in the range of 2.5-25μg/mL-1 with regression coefficient 0.999. Intraday precision studies showed the relative standard deviation ≤1.58% and  inter-day ≤3.20%. Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was 102.18%. The Limit of Detection Limit of Quantitation (LOQ) and were 0.47 and 1.56μg/mL-1.The contents of rubraxanthon in the crude was 0.23±0.07%w/w.


Full Text:

PDF (PP.237-244)

References


Dachriyanus MI., Rizal F., 2003, Antimicrobial and Antioxidant Activity Test Compound Results Isolation of Skin Stem Plants Garcinia cowa Roxb. J. Math. Nat Sci. 12: 67-72

Feldine P., Abeyta C., Andrews WH., 2002, AOAC International methods committee guidelines for validation of qualitatitive and quantitative food microbiological official methods of analysis, J AOAC Int., 85: 1-5

Hefnawy MM., Sultan MA., Al-Shehri MM. 2006. Direct enantiomeric resolution of betaxolol with application to analysis of pharmaceutical products. Anal. Chem. Insights. 1: 13-20.

Iimuna M., Tosa H., Tanaka T., Asai F., Kobayashi Y., Shimano R. 1996. Antibacterial Activity of Xanthones from Guttiferous Plants Against Methicillin - Resistant Staphylococcus aureus. J. Pharm. Pharmacol. 48 : 861-865

International converence on Harmonization of note for Guidence on validation of analytical procedures, text and methodology. Step 5 of ICH. 1995

Jantan I., Pisar M., Idris Md.MS., Taher M., Ali RM.. 2002. In vitro Inhibitory Effect of Rubraxanthone Isolated from Garcinia parvifolia on Platelet-Activating Factor Receptor Binding. Planta Med. 68: 1133-1134

Jayaprakasha GK., Negi PS., Jena BS. 2006. Antioxidative and antimutagenic activities of the extracts from the rinds of Garcinia pendunculata. Innovative Food Science and Emerging Technologies. 7: 246-250

Pattalung P., Wiriyachitra P., Ongsakol M. 1987. The antimicrobial activities of rubraxanthone isolated from Garcinia parvifolia (Miq). J. Sci. Soc.Thailand. 14: 67-75

Patra KC., Pareta SK., Harwansh RK., Kumar KJ. 2010. Traditional approaches towards standardization of herbal medicines-A review. J Pharm Sci Technol 2: 372-379.

Shinde VM., Dhalwal K., Potdar M., Mahadik, KR. 2009. Application of quality control principles to herbal drugs. Int. J. Phytomed 1 :4




DOI: http://dx.doi.org/10.14499/indonesianjpharm25iss4pp237

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

web
analytics View My Stats