CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESIS

Gabriel Hancu, Monica Budău, Lajos Attila Papp, Aura Rusu, Eleonora Mircia

Abstract


Chiral separation cetirizine, a second generation H1 antagonist was studied by cyclodextrine (CD) mediated capillary electrophoresis. The influence on the separation of several parameters including pH and concentration of the background electrolyte (BGE), CD type and concentration, applied voltage and temperature were studied and the electrophoretic and analytic parameters were optimized. The best conditions for the chiral separation were obtained using 25mM disodium hydrogeno-phosphate – 25mM sodium didydrogeno-phosphate (1:1) as BGE, 5mM sulfobuthyl ether- β-CD as chiral selector, a voltage of + 20kV, temperature of 20°C, injection pressure/time of 50mbar/ 1sec, UV detection at 230nm. The analytical performance of the method was evaluated. The proposed method was successfully applied to the enantioselective assay of cetirizine in pharmaceutical formula-tions. CE proved to be a rapid, specific, reliable and cost-effective method for the chiral separation of cetirizine enantiomers and can be useful for laboratories performing routine analysis.

 


Full Text:

PDF (PP.223-229)

References


Block JH., Beale JM. 2011. Wilson and Gisvold’s textbook of Organic Medicinal and Pharmaceutical Chemistry. 12th ed. Lippincott Williams & Wilkins, Philadephia, USA. pp. 755-756.

Chou YW., Huang WS., Ko CC, Chen SH. 2008. Enantioseparation of cetirizine by sulfated-β-cyclodextrin-mediated capillary electrophoresis. J. Sep. Sci. 31: 845–852.

Fanali S. 2000. Enantioselective determination by capillary electrophoresis with cyclodextrins as chiral selectors. J. Chromatogr. A. 875: 89-122.

Fillet M., Hubert P., Crommen J. 1998. Method development strategies for the enantioseparation of drugs by capillary electrophoresis using cyclodextrins as chiral additives. Electrophoresis. 19: 2834-2840.

Gubitz G., Schmid MG. 1997. Chiral separation principles in capillary electrophoresis. J. Chromatogr. A. 792: 179-225.

Gubitz G., Schmid MG. 2008. Chiral separation by capillary electromigration techniques. J. Chromatogr. A. 1204: 140-156.

Martindale. 2011. The Complete Drug Reference. 37th ed. Pharmaceutical Press, London, UK. pp 570-571.

Mikus P., Valaskova I., Havranek E. 2005. Enantioselective analysis of cetirizine in pharmaceuticals by cyclodextrin-mediated capillary electrophoresis. J. Sep. Sci. 28: 1278–1284.

Nojavan S., Fakhari AR. 2011. Chiral separation and quantitation of cetirizine and hydroxyzine by maltodextrin-mediated CE in human plasma: Effect of zwitterionic property of cetirizine on enantioseparation. Electrophoresis. 32: 764-771.

Ren X., Dong Y., Liu J., Huang A., Liu H., Sun, Y., Sun Z. 1999. Separation of chiral basic drugs with sulfobutyl- β-cyclodextrin in capillary electrophoresis. Chromatographia. 50: 363-368.

Van Eeckhaut A., Michotte Y. 2006. Chiral separation of cetirizine by capillary electrophoresis. Electrophoresis. 27: 2376–2385.




DOI: http://dx.doi.org/10.14499/indonesianjpharm25iss4pp223

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

                                    
 
web
analytics View My Stats