Naringenin-Loaded TPGS Polymeric Nanosuspension: In-Vitro and In-Vivo Anti-Inflammatory Activity

Sumathi Rajamani, Tamizharasi Sengodan, Sivakumar Thangavelu, Nikhitha K Shanmukhan, Arun Radhakrishnan

Abstract


Naringenin, (NAR) from Citrus grandis(L.) Osbeck, family Rutaceae, exhibit extensive pharmacological action, lacks significance in application due to low aqueous solubility approximately  0.214 mg/ml, which results in low bioavailability of 5.8%. Nanosuspension of NAR (NARNS) was prepared in our previous studies using high pressure homogenization adding various polymers. All these formulations were characterized and as a continuation of our work formulations was further evaluated for their anti-inflammatory activity by in-vitro and in-vivo methods. Denaturation of protein method and membrane stabilization methods were chosen for in-vitro evaluation. In-vivo studies performed were acute inflammatory studies (carrageenan-induced paw oedema) and chronic inflammatory studies (cotton pellet granuloma) on Wistar albino rats. The studies demonstrated that the NAR and NARNS at a dose of 50 mg/kg P.O. have a potent activity compared to the standard drug diclofenac.  The percentage protection against inflammation exhibited by NARNS was highly significant compared to NAR.


Keywords


Carrageenan, oxidative stress, anti-inflammatory, Naringenin

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DOI: http://dx.doi.org/10.14499/indonesianjpharm30iss3pp225

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