Antibacterial Compound from Aspergillus elegans SweF9 an Endophytic Fungus from Macroalgae Euchema sp.

Hani Mulyani, Rizna Triana Dewi, Chaidir Chaidir

Abstract


The antibiotic resistance of bacterial pathogens has become a serious health concern and encouragement to search for novel and efficient antimicrobial metabolites. On the other hand, endophytic fungi have great potential as a natural source for antimicrobial agents. The objective of this study was to isolation antibacterial compound from endophytic fungi of A.elegans SweF9. The fungus was stationarily cultured at 30°C for 12 days in potato malt peptone (PMP) medium, then extracted with ethyl acetate. The antibacterial activities of the extract were evaluated by agar well diffusion method against Gram-poitive (Bacillus subtilis and  Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains. The broth extract was able to inhibit the growth of E. coli, S. aureus and B. subtilis with antibacterial activity index compared to streptomycin sulfate were 84.6%, 91.6%, and 90% respectively. The active compound (1) was purified to yield amorphous white and identified using FTIR, NMR, and EI-MS analyses, revealed identified as (+) - epi-Epoformin. The compound showed an antibacterial activity index of E. coli, S. aureus and B. subtilis  bacterial were 38%, 45%, and 47%, respectively.  Based on these results endophytic fungi A. elegans SweF9 can be used as a new source of potential antibacterial compounds

Keywords


Aspergillus elegans SweF9, antibacteria, (+)-epi-Epoformin.

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References


Barilli E, Cimmino A, Masi M, Evidente M, Rubialesa D, and Evidente A. 2016. Inhibition of early developpment stages of rust fungi by the two fungal metabolites cyclopaldicacid and epi-epoformin. Pest Manag Sci.: 1-9

Cala A, Masi M, Cimmino A, Molinillo JMG. Marcias FA, Evidente A. 2018. (+)-epi-epoformin, a phytotoxic fungal cyclohexenepoxide: structure activity relationships. Molecules. 23: 1-12.

Choma IM and Grzelak EM. 2015. TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants. Chromatography. 2: 225-238.

Elisha IL, Botha FS, McGaw LJ, Eloff JN. 2017. The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complement Altern Med. 17 (133): 1-10.

Jesionek W, Alberti A, Ott GP, Kocsis B, Horváth G, Choma IM. 2015. TLC-Direct Bioautography as a Bioassay Guided Method for Investigation of Antibacterial Compounds in Hypericum perforatum L. Journal of AOAC International. 98 (4): 1013-1020.

Kalyanasundaram I, Nagamuthu J, Muthukumaraswamy S. 2015. Antimicrobial activity of endophtic fungi isolated and identified from salt marsh in Vellar Estuary. Academic Journals.7: 13-20.

Nagasawa H, Suzuki A, Tamura S. 1978. Isolation and structure of (+)-des-oxyepiepoxydon and (+)-epi-epoxydon, phytotoxic fungal metabolites. Agric. Biol. Chem. 42: 1303–1304.

Rateb ME and Ebel R. 2011. Secondary metabolites of fungi from marine habitats. Nat. Prod. Rep. 28: 290–344.

Santos IP, Silva LCN, Silva MV, Araújo JM, Cavalcanti MS and Lima VLM. 2015. Antibacterial activity endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae). Front. Microbiol. 6:350.

Sarasan M, Puthumana J, Neema Job N, Han J, Lee JS, and Philip R. 2017. Marine Algicolous Endophytic Fungi - A Promising Drug Resource of the Era. J. Microbiol. Biotechnol. 27(6), 1039–1052.

Thomas TR, Kavlekar DP, LokaBharathi PA. 2010. Marine drugs from sponge–microbe association—A review. Mar. Drugs. 8: 1417–1468.

Yoice S and Desak SGA. 2015. Preliminary of Antibacterial Activity from Indonesia Marine-Derived Endophytic Fungi. Adv Environ Biol. 9(9): 143-149.

Zainuddin N, Alias SA, Lee CW, Ebel R, Othman NA, Mukhtar MR, Awang K. 2010. Antimicrobial activities of marine fungi from Malaysia. Botanica Marina 53: 507–513




DOI: http://dx.doi.org/10.14499/indonesianjpharm30iss3pp217

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