Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis

Evi Istiqamah, Dicky Moch. Rizal, Ika Puspitasari

Abstract


Testicular damage is a serious complication of diabetes mellitus resulting in male infertility, which is associated with caspase-3 and IGF-1 mRNA expression. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testes for diabetes mellitus and there are no studies in the literature concerning the influence of PRP on expressions of growth factors in testes.

The aim of this study was to investigate the efficacy of adjunctive PRP in insulin treatment for repair of testicular damage in a diabetic rat model. Diabetes was induced by administering single dose 60 mg/kg streptozotocin. Twenty Wistar male rats were divided into four groups: group 1, control group; group 2, diabetes without treatment; group 3, diabetes with treated insulin; and group 4, diabetes with treated insulin and PRP. Rats were euthanized after two weeks of treatment, and testes were taken for caspase-3 and IGF-1 mRNA expression measurements.

Diabetes mellitus induction caused a significant increase in caspase-3 mRNA expression with p=0.049 and significant decrease in IGF-1 mRNA expression with p=0.004. There was no difference in caspase-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to without PRP.


Keywords


PRP, diabetic rat testis, caspase-3 mRNA expression, IGF-1, mRNA in expression

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References


Bakacak M., Bostanci MS., İnanc F., Yaylali A., Serin S., Attar R.., et al., 2015. Protective effect of platelet rich plasma on experimental ischemia/reperfusion injury in rat ovary. Gynecol. Obstet. Invest. 81: 225–231.

Barrett EJ., 2017. Endocrine regulation of growth and body mass. In: Boron WF., Boulpaep EL (Ed.): Medical Physiology. 3rd ed. Elsevier, Philadelphia. pp. 990–1005

Bonert VS., Melmed S. (Ed.), 2017. Growth hormone. The Pituitary. 4th ed. Elsevier, London. pp. 85–127

Bronson RA., 1999. Antisperm antibodies: A critical evaluation and clinical guidelines. J. Reprod. Immunol. 45: 159–183.

Carver C., 2006. Insulin treatment and the problem of weight gain in type 2 diabetes. Diabetes Educ. 32: 910–917.

Castilla-Cortázar I., Gago A., Muñoz Ú., Ávila-Gallego E., Guerra-Menéndez L., Sádaba MC., García-Magariño M., et al., 2015. Mechanisms underlying testicular damage and dysfunction in mice with partial IGF-1 deficiency and the effectiveness of IGF-1 replacement therapy. Urology 86: 1241–1250.

Chemaitilly W., Sklar CA., 2007. Endocrine complications of hematopoietic stem cell transplantation. Endocrinol. Metab. Clin. North Am. 36: 983–998.

Devereaux J., Nurgali K., Kiatos D., Sakkal S., Apostolopoulos V., 2018. Effects of platelet-rich plasma and platelet-poor plasma on human dermal fibroblasts. Maturitas 117: 34–44.

Dhillon RS., Schwarz EM., Maloney MD., 2012. Platelet-rich plasma therapy - future or trend? Arthritis Res. Ther. 14: 219–229.

Dimitriadis G., Mitrou P., Lambadiari V., Maratou E., Raptis SA., 2011. Insulin effects in muscle and adipose tissue. Diabetes Res. Clin. Pract. 93: S52–S59.

El-Sharkawy H., Kantarci A., Deady J., Hasturk H., Liu H., Alshahat M., et al., 2007. Platelet-rich plasma: Growth factors and pro- and anti-Inflammatory properties. J. Periodontol. 78: 661–669.

Fitzpatrick J., Bulsara MK., McCrory PR., Richardson MD., Zheng MH., 2017. Analysis of platelet-rich plasma Extraction. Orthop. J. Sport. Med. 5: 2325967116675272.

Gandhi A., Doumas C., O’Connor JP., Parsons JR., Lin SS., 2006. The effects of local platelet rich plasma delivery on diabetic fracture healing. Bone. 38: 540–546.

Kang S., Song J., Kang H., Kim S., Lee Y., Park D., 2003. Insulin can block apoptosis by decreasing oxidative stress via phosphatidylinositol 3-kinase- and extracellular signal-regulated protein kinase-dependent signaling pathways in HepG2 cells. Eur. J. Endocrinol. 148: 147–155.

Kersten S., 2001. Mechanisms of nutritional and hormonal regulation of lipogenesis. EMBO Report. 2: 282–286.

Kianifard D., Sadrkhanlou RA., Hasanzadeh S., 2012. The ultrastructural changes of the Sertoli and Leydig cells following streptozotocin induced diabetes. Iran. J. Basic Med. Sci. 15: 623–635.

Koksal IT., Ishak Y., Usta M., Danisman A., Guntekin E., Bassorgun IC., et al., 2007. Varicocele-induced testicular dysfunction may be associated with disruption of blood-testis barrier. Arch. Androl. 53: 43–48.

Kong W-Y., Tong L-Q., Zhang H-J., Cao Y-G., Wang G-C., Zhu J-Z., et al., 2016. The calcium-sensing receptor participates in testicular damage in streptozotocin-induced diabetic rats. Asian J. Androl. 18: 803–808.

Levy GM., Lucas P., Hope N., 2018. Efficacy of a platelet-rich plasma injection for the treatment of proximal hamstring tendinopathy: A pilot study. J. Sci. Med. Sport. 22: 247–252.

Lyras DN., Kazakos K., Agrogiannis G., Verettas D., Kokka A., Kiziridis G., et al., 2010. Experimental study of tendon healing early phase: Is IGF-1 expression influenced by platelet rich plasma gel? Orthop. Traumatol. Surg. Res. 96: 381–387.

Martínez-Zapata MJ., Martí-Carvajal A., Solà I., Bolibar I., Ángel Expósito J., Rodriguez L., et al., 2009. Efficacy and safety of the use of autologous plasma rich in platelets for tissue regeneration: A systematic review. Transfusion. 49: 44–56.

Morsy M., Ramzy M., El-Sheikh AK., Kamel M., Abdelwahab S., 2014. Mechanism of testicular protection of carvedilol in streptozotocin-induced diabetic rats. Indian J. Pharmacol. 46: 161–165.

Nambam B., Schatz D., 2018. Growth hormone and insulin-like growth factor-I axis in type 1 diabetes. Growth Horm. IGF Res. 38: 49–52.

Olchosky D., Bruno JF., Gelato MC., Song J., dan Berelowitz M., 1991. Pituitary insulin-like growth factor-I content and gene expression in the streptozotocin-diabetic rat: Evidence for tissue-specific regulation*. Endocrinology 128: 923–928.

Ozkurkcugil C., Yardimoglu M., Dalcik H., Erdogan S., Gokalp A., 2004. Effect of insulin-like growth factor-1 on apoptosis of rat testicular germ cells induced by testicular torsion. BJU Int. 93: 1094–1097.

Puche JE., Castilla-Cortázar I., 2012. Human conditions of insulin-like growth factor-I (IGF-I) deficiency. J. Transl. Med. 10: 224.

Said TM., 2004. Role of caspases in male infertility. Hum. Reprod. Update. 10: 39–51.

Sekerci CA., Tanidir Y., Sener TE., Sener G., Cevik O., Yarat A., et al., 2017. Effects of platelet-rich plasma against experimental ischemia/reperfusion injury in rat testis. J. Pediatr. Urol. 13: 317.e1-317.e9.

Shi G., Li Z-M., Zheng J., Chen J., Han X., Wu J., et al., 2017. Diabetes associated with male reproductive system damages: Onset of presentation, pathophysiological mechanisms and drug intervention. Biomed. Pharmacother. 90: 562–574.

Shi G-J., Zheng J., Wu J., Qiao H-Q., Chang Q., Niu Y., et al., 2017. Beneficial effects of Lycium barbarum polysaccharide on spermatogenesis by improving antioxidant activity and inhibiting apoptosis in streptozotocin-induced diabetic male mice. Food Funct. 8: 1215–1226.

Sisman AR., Kiray M., Camsari UM., Evren M., Ates M., Baykara B., et al., 2014. Potential novel biomarkers for diabetic testicular damage in streptozotocin-induced diabetic rats: Nerve growth factor beta and vascular endothelial growth factor. Dis. Markers: 2014: 108106.

Takase F., Inui A., Mifune Y., Sakata R., Muto, T., Harada Y., et al., 2017. Effect of platelet-rich plasma on degeneration change of rotator cuff muscles: In vitro and in vivo evaluations. J. Med. Virol. 35: 1806–1815.

Wang G., Hardy MP., 2004. Development of Leydig cells in the insulin-like growth factor-I (IGF-I) knockout mouse: Effects of IGF-I replacement and gonadotropic stimulation. Biol. Reprod. 70: 632–639.

Wasterlain AS., Braun HJ., Harris AHS., Kim H., Dragoo JL., 2013. The systemic effects of platelet-rich plasma injection. Am. J. Sports Med. 41: 186–193.

Wolfe RR., 2000. Effects of insulin on muscle tissue. Curr. Opin. Clin. Nutr. Metab. Care. 3: 67–71.

Wu PIK., Diaz R., Borg-Stein J., 2016. Platelet-rich plasma. Phys. Med. Rehabil. Clin. N. Am. 27: 825–853.

Yoon MJ., Roser JF., 2010. Insulin-like growth factor-I (IGF-I) protects cultured equine Leydig cells from undergoing apoptosis. Anim. Reprod. Sci. 122: 353–358.




DOI: http://dx.doi.org/10.14499/indonesianjpharm30iss2pp98-104

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