Immunomodulatory Activity of Yogurt Fortified with Honey and Rosella (Hibiscus sabdariffa L.) on Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO) Secretion

Nurkhasanah Mahfudh, Rina Novitasari

Abstract


Raise the body's immune system. Rosella (Hibiscus sabdariffa Linn.) is known to have anthocyanin compounds that have antioxidant and immunomodulatory effects. The aim of this research is to know the effect of immunomodulator yogurt which fortified by rosella (Hibiscus sabdariffa L.) extract to increase the secretion of Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO). This research was conducted in vivo using 25 male mices with Balb/C strain divided into 5 groups: normal group, plain yogurt treated group and 2%, 4%, 8% rosella yogurt treated group. Treatment was given for 21 days orally. On the 22nd day the mice were sacrificed by taking peritoneum macrophage cells and then tested the secretion of Reactive Oxygen Intermediate (ROI) and Nitrite Oxide (NO). The results showed that there was an increase of ROI and NO secretion in 2%, 4%, and 8% rosella yoghurt treated groups compare to plain yoghurt. The fortification of yoghurt with rosella extract and honey increase the potency of yoghurt in increasing the immunomodulatory activity

Keywords


Immunomodulator, Yoghurt, Rosella (Hibiscus sabdariffa L), ROI, NO

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References


Abbas, A., Lichtman, A. H., & Pilla, S. (2017). Cellular and Molecular Immunology (9th ed.). Elsevier.

Akrom, A., Widjaya, A., & Armansyah, T. (2015). Extract of Black Cumin (Nigella sativa) Seed Increases Macrophage Phagocytic Activity of Swiss Mice Infected with Lysteria monocytogenes. Jurnal Kedokteran Hewan, 9(2), 94–100.

Astawan, M., Wresdiyati, T., Arief, I. I., & Febiyanti, D. (2011). Potency of Indigenous Probiotic Lactic Acid Bacteria as Antidiarrheal Agent and Immunomodulator. Jurnal Teknologi Dan Industri Pangan, XXII(1), 11–16.

Baratawidjaya. (2006). Imunologi Dasar. Jakarta: fakultas Kedokteran Universitas Indonesia.

Chiu, C., Chen, J., Chou, F., & Lin, H. (2015). Hibiscus sabdariffa Leaf Extract Inhibits Human Prostate Cancer Cell Invasion via Down-Regulation of Akt/NF-(B/MMP-9 Pathway. Nutrients, 7, 5065–5087. https://doi.org/10.3390/nu7075065

Fakeye, T. (2008). Toxicity and immunomodulatory activity of fractions of Hibiscus sabdariffa Linn (family Malvaceae) in animal models. African Journal of Traditional Complementary and Alternative Medicine.

Herrera-Arellano, A., Miranda-Sánchez, J., Ávila-Castro, P., Herrera-Álvarez, S., Jiménez-Ferrer, J. E., Zamilpa, A., … Tortoriello, J. (2007). Clinical effects produced by a standardized herbal medicinal product of Hibiscus sabdariffa on patients with hypertension. A randomized, double-blind, lisinopril-controlled clinical trial. Planta Medica, 73(1), 6–12. https://doi.org/10.1055/s-2006-957065

Hirunpanich, V., Utaipat, A., Morales, N. P., Bunyapraphatsara, N., Sato, H., Herunsalee, A., & Suthisisang, C. (2005). Antioxidant effects of aqueous extracts from dried calyx of Hibiscus sabdariffa Linn. (Roselle) in vitro using rat low-density lipoprotein (LDL). Biological & Pharmaceutical Bulletin, 28(3), 481–484. https://doi.org/10.1248/bpb.28.481

Lima, A. D. J. B., Corrêa, A. D., Saczk, A. A., Martins, M. P., & Castilho, R. O. (2011). Anthocyanins , pigment stability and antioxidant activity in jabuticaba [ myrciaria cauliflora ( mart .) O . Berg ]. Rev. Bras. Fruit, 33(3), 877–887.

Nurkhasanah, Santoso, D. R., & Fauziah, R. (2017). The immunomodulatory effect of Zingiber cassumunar ethanolic extract on phagocytic activity , nitrit oxide and reaxtive oxygen intermediate secretions of macrophage in mice. IOP Conf. Series: Materials Science and Engineering, 259(2017), 1–10. https://doi.org/10.1088/1757-899X/259/1/012007

Nurkhasanah, & Zulkarmen, L. R. (2014). Efek ekstrak etanol kelopak rosella (Hibiscus sabdariffa, L.) terhadap sekresi nitrit oksida (NO) makrofag peritoneum tikus yang diinduksi 7,12-dimethylbenz(a)antracene (DMBA). Media Farmasi, 11(2), 155–166.

Usoh, I. F., Akpan, E. J., Etim, E. O., & Farombi, E. O. (2005). Antioxidant Actions of Dried Flower Extracts of Hibiscus sabdariffa L . On Sodium Arsenite - Induced Oxidative Stress in Rats, 4(3), 135–141.

Widodo, W. (2002). Bioteknologi fermentasi susu. Malang: Pusat Pengembangan Bioteknologi, Universitas Muhammadiyyah Malang.




DOI: http://dx.doi.org/10.14499/indonesianjpharm30iss2pp141-146

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