The Properties of Brown Marine Algae Sargassum turbinarioides and Sargassum ilicifolium Collected From Yogyakarta, Indonesia

Rahma Artemisia; Akhmad Kharis Nugroho; Erna Prawita Setyowati; Ronny Martien

doi:10.14499/indonesianjpharm30iss1pp43

The Properties of Brown Marine Algae Sargassum turbinarioides and Sargassum ilicifolium Collected From Yogyakarta, Indonesia

Rahma Artemisia, Akhmad Kharis Nugroho, Erna Prawita Setyowati, Ronny Martien

Abstract

Brown marine algae are the prominent source of marine natural products that have bioactive metabolites. Sargassum turbinarioides and Sargassum ilicifolium were dominated in Indonesia as brown marine algae that well known as a source of fucoidan. The samples were collected from Gunungkidul, Yogyakarta, Indonesia. In this study, we investigated and identified the yield of aqueous crude and purified extracts using different extraction temperatures (60°C, 70°C, 80°C, 90 °C). The highest yield of S. turbinarioides crude extract (7.36%) was obtained at temperatures 90 and 80 °C while the highest yield of S. ilicifolium was 3.49 % at 80 °C. The presence of sulfate polysaccharide in Sargassum turbinarioides is 3,78 % and Sargassum ilicifolium is 2,93 %. Each of the extract was screened using phytochemical detection, Thin Layer Chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR) analysis. The phytochemical detection indicated that Sargassum ilicifolium has bioactive metabolites such as carbohydrates, proteins and amino acids, terpenoid, phenolic compounds, and flavonoids. The FTIR spectrum of the S. turbinarioides and Sargassum ilicifolium extract refer to the presence of ester sulfate groups through showing peaks at 1300 to 1200 cm^-1 and 980 to 950 cm-1. The result indicated that Sargassum turbinarioides and Sargassum ilicifolium contain of sulfate polysaccharide were prospect a biological activities to use for the development of marine nutraceutical drugs especially as antioxidant.

Keywords

Sargassum turbinarioides, Sargassum ilicifolium, Fucoidan, Thin Layer Chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy

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References

Ahmed, A. B. A., Adel, M., Karimi, P., & Peidayesh, M. (2014). Pharmaceutical, Cosmeceutical, and Traditional Applications of Marine Carbohydrates. In Advances in Food and Nutrition Research (Vol. 73, pp. 197–220). Elsevier. Retrieved from http://linkinghub.elsevier.com/retrieve/pii/B978012800268100010X

Ale, M. T., Maruyama, H., Tamauchi, H., Mikkelsen, J. D., & Meyer, A. S. (2011). Fucoidan from Sargassum sp. and Fucus vesiculosus reduces cell viability of lung carcinoma and melanoma cells in vitro and activates natural killer cells in mice in vivo. International Journal of Biological Macromolecules, 49(3), 331–336. https://doi.org/10.1016/j.ijbiomac.2011.05.009

Berthomieu, C., & Hienerwadel, R. (2009). Fourier transform infrared (FTIR) spectroscopy. Photosynthesis Research, 101(2–3), 157–170. https://doi.org/10.1007/s11120-009-9439-x

Chranioti, C., Chanioti, S., & Tzia, C. (2016). Comparison of spray, freeze and oven drying as a means of reducing bitter aftertaste of steviol glycosides (derived from Stevia rebaudiana Bertoni plant) – Evaluation of the final products. Food Chemistry, 190, 1151–1158. https://doi.org/10.1016/j.foodchem.2015.06.083

Cunha, L., & Grenha, A. (2016). Sulfated Seaweed Polysaccharides as Multifunctional Materials in Drug Delivery Applications. Marine Drugs, 14(3), 42. https://doi.org/10.3390/md14030042

Dhinakaran, D. I., Rajalakshmi, R., Sivakumar, T., & Jeeva, S. (2016). Antimicrobial Activities and Bioactive Metabolites from Marine Algae Valoniopsis pachynema and Sargassum swartzii. Journal of Pharmacognosy and Phytochemistry, 4(1).

Fleurence, J. (2016). Seaweed in health and disease prevention. Boston, MA: Elsevier.

Halling, B., Vetvicka, V., & Blakemore, W. . (2015). Evaluation of The Immunomodulatory in vivo Activity of Laminaria Hyperborea Fucoidan Relative to Commercial (1,3/1,6)-Β-D-Glucans from Yeast and Mushrooms. Journal of Nutrition and Health Sciences, 2(2). https://doi.org/10.15744/2393-9060.2.205

Holtkamp, A. D., Kelly, S., Ulber, R., & Lang, S. (2009). Fucoidans and fucoidanases—focus on techniques for molecular structure elucidation and modification of marine polysaccharides. Applied Microbiology and Biotechnology, 82(1), 1–11. https://doi.org/10.1007/s00253-008-1790-x

Indrawati, R., Sukowijoyo, H., Indriatmoko, Wijayanti, R. D. E., & Limantara, L. (2015). Encapsulation of Brown Seaweed Pigment by Freeze Drying: Characterization and its Stability during Storage. Procedia Chemistry, 14, 353–360. https://doi.org/10.1016/j.proche.2015.03.048

Jolly, R. R., & Iyer, P. (2015). Isolation, Characterization and Application of Fucoidan from Weeds. Nternational Journal of Current Research in Biosciences and Plant Biology, 2(7), 212–216.

Kusaykin, M., Bakunina, I., Sova, V., Ermakova, S., Kuznetsova, T., Besednova, N., … Zvyagintseva, T. (2008). Structure, biological activity, and enzymatic transformation of fucoidans from the brown seaweeds. Biotechnology Journal, 3(7), 904–915. https://doi.org/10.1002/biot.200700054

Li, B., Wei, X.-J., Sun, J.-L., & Xu, S.-Y. (2006). Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikia fusiforme. Carbohydrate Research, 341(9), 1135–1146. https://doi.org/10.1016/j.carres.2006.03.035

Lim, S. J., Wan Aida, W. M., Maskat, M. Y., Mamot, S., Ropien, J., & Mazita Mohd, D. (2014). Isolation and antioxidant capacity of fucoidan from selected Malaysian seaweeds. Food Hydrocolloids, 42, 280–288. https://doi.org/10.1016/j.foodhyd.2014.03.007

Lim, S. N., Cheung, P. C. K., Ooi, V. E. C., & Ang, P. O. (2002). Evaluation of Antioxidative Activity of Extracts from a Brown Seaweed, Sargassum siliquastrum. Journal of Agricultural and Food Chemistry, 50(13), 3862–3866. https://doi.org/10.1021/jf020096b

Manoj, S. G. M., Mahesh, K. P. S., Vasanthi, M., & Anant, A. (2013). Anticoagulant property of sulphated polysaccharides extracted from marine brown algae collected from Mandapam Island, India. African Journal of Biotechnology, 12(16), 1937–1945. https://doi.org/10.5897/AJB2013.12109

Patankar, M. S., Oehninger, S., Barnett, T., Williams, R. L., & Clark, G. F. (1993). A revised structure for fucoidan may explain some of its biological activities. Journal of Biological Chemistry, 268, 21770–21776.

Pérez, M. J., Falqué, E., & Domínguez, H. (2016). Antimicrobial Action of Compounds from Marine Seaweed. Marine Drugs, 14(3), 52. https://doi.org/10.3390/md14030052

Pielesz, A., & Biniaś, W. (2010). Cellulose acetate membrane electrophoresis and FTIR spectroscopy as methods of identifying a fucoidan in Fucus vesiculosus Linnaeus. Carbohydrate Research, 345(18), 2676–2682. https://doi.org/10.1016/j.carres.2010.09.027

Ponce, N. M. A., Pujol, C. A., Damonte, E. B., Flores, M. L., & Stortz, C. A. (2003). Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies. Carbohydrate Research, 338(2), 153–165. https://doi.org/10.1016/S0008-6215(02)00403-2

Raghavendran, H. R. B., Srinivasan, P., & Rekha, S. (2011). Immunomodulatory activity of fucoidan against aspirin-induced gastric mucosal damage in rats. International Immunopharmacology, 11(2), 157–163. https://doi.org/10.1016/j.intimp.2010.11.002

Rohman, A., Che Man, Y. B., Ismail, A., & Puziah, H. (2011). FTIR spectroscopy combined with multivariate calibration for analysis of cod liver oil in binary mixture with corn oil. International Food Research Journal, 18(2).

Senthilkumar, K., Manivasagan, P., Venkatesan, J., & Kim, S.-K. (2013). Brown seaweed fucoidan: Biological activity and apoptosis, growth signaling mechanism in cancer. International Journal of Biological Macromolecules, 60, 366–374. https://doi.org/10.1016/j.ijbiomac.2013.06.030

Sinurat, E., Peranginangin, R., & Saepudin, E. (2016). Purification and Characterization of Fucoidan from the Brown Seaweed Sargassum binderi Sonder. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, 10(2), 79. https://doi.org/10.15578/squalen.v10i2.133

Sugiono, Widjanarko, S. B., & Soehono, L. A. (2014). Extraction Optimization by Response Surface Methodology and Characterization of Fucoidan from Brown Seaweed Sargassum polycystum. International Journal of ChemTech Research, 6(1), 195–205.

Thuy, T. T. T., Ly, B. M., Van, T. T. T., Van Quang, N., Tu, H. C., Zheng, Y., … Ai, U. (2015). Anti-HIV activity of fucoidans from three brown seaweed species. Carbohydrate Polymers, 115, 122–128. https://doi.org/10.1016/j.carbpol.2014.08.068

Wang, C.-Y., Wu, T.-C., Hsieh, S.-L., Tsai, Y.-H., Yeh, C.-W., & Huang, C.-Y. (2015). Antioxidant activity and growth inhibition of human colon cancer cells by crude and purified fucoidan preparations extracted from Sargassum cristaefolium. Journal of Food and Drug Analysis, 23(4), 766–777. https://doi.org/10.1016/j.jfda.2015.07.002

Wijesinghe, W. a. J. P., & Jeon, Y.-J. (2012). Biological activities and potential industrial applications of fucose rich sulfated polysaccharides and fucoidans isolated from brown seaweeds: A review. ResearchGate, 88(1), 13–20. https://doi.org/10.1016/j.carbpol.2011.12.029

Zhao, X., Xue, C.-H., & Li, B.-F. (2008). Study of antioxidant activities of sulfated polysaccharides from Laminaria japonica. Journal of Applied Phycology, 20(4), 431–436. https://doi.org/10.1007/s10811-007-9282-4

Zvyagintseva, T. N., Shevchenko, N. M., Popivnich, I. B., Isakov, V. V., Scobun, A. S., Sundukova, E. V., & Elyakova, L. A. (1999). A new procedure for the separation of water-soluble polysaccharides from brown seaweeds. Carbohydrate Research, 322(1–2), 32–39. https://doi.org/10.1016/S0008-6215(99)00206-2

DOI: http://dx.doi.org/10.14499/indonesianjpharm30iss1pp43