Effects of Probiotics and Vitamin B Supplementation on IFN-γ and IL-12 Levels During Intensive Phase Treatment of Tuberculosis
Tuberculosis is an acute infectious disease that primarily affects the lungs. Probiotics supplementation can increase the number and activity of NK cell in peripheral blood by modulation of IL-12, thus increasing IFN-γ production by Th1 response. Vitamin B1 acts on macrophages and affects neutrophil motility. Vitamin B6 is associated with the release of cytokines and the responsiveness of NK cells, while vitamin B12 affects to lymphocytes, Tcell proliferation, CD4+ ratios, and NK cell activity. To analyze the effects of probiotics and vitamin B1, B6, B12 supplementation on IFN-γ and IL-12 levels during intensive phase of antituberculosis treatment. The study was pre-post test randomised control by time series. The control group was TB patients with standard therapy of antituberculosis and vitamin B6, while the intervention group was TB patients receiving therapy plus once daily probiotics and vitamin B1, B6, B12supplementation during the intensive phase. Blood samples were withdrawn at baseline, one month, and two months after therapy to measure plasma IFN-γ and IL-12 levels using the ELISA method. Twenty two patients were divided equally into two groups. There was a tendency to greater increase of IFN-γ in the first month of the intervention group, followed by a significant decline after two-month therapy (p < 0.05). In both groups there was a rise in IL-12 levels after one month followed by a decrease in the second month (p > 0.05). However, the percentage was higher in the supplementation group. Adding probiotics and vitamins B1, B6, B12 could improve immune response through IL-12 and IFN-γ modulation during intensive phase therapy.
Abul K. Abbas, Andrew H. H. Lichtman, Shiv Pillai. 2016, ‘Basic Immunology : Functions and Disorders of the Immune System’. Elsevier Inc. Ed.5. pp 27-52
Adrian, T.B. R., Montiel, J.L., Fernandez, G., Valecillo, A. 2015. “Role of Cytokines and other Factors Involved in the Mycobacterium Tuberculosis Infection”. World Journal of Immunology. Vol 5 (1). pp 16 – 50
Chapman, C.M.C., Gibson, G.R & Rowland, I. 2011. “Health Benefits of Probiotics : Are Mixture More Effective than Single Strains ?” European Journal of Nutrition. 50(1). 1-17.
Chapman, C.M.C., Gibson, G.R & Rowland, I. 2012. “In Vitro Evaluation of Single- and Multi-Strain Probiotics : Inter-Species Inhibition Between Probiotic Strains, and Inhibition of Pathogens”. Anaerobe. 18. 405 – 413.
Clifford, V., Zufferey, C., Street, A., Denholm, J., Tebruegge, M., Curtis, N., 2015. “Cytokines for monitoring anti-tuberculous therapy: A systematic review”. Journal Tuberculosis. Vol 95 (3). pp 217-228
Deveci, F., Akbulut, H.H., Turgut, T., Muz, H.M. 2005. “Changes in Serum Cytokine Levels in Active Tuberculosis With Treatment”. Mediators of Inflammmation. 5. 256-262.
Etna, M.P., Giacomini, E., Severa,M., and Coccia, E.M, 2014. Pro- and Anti-Inflammatory Cytokines in TB: A Two-Edged Sword in TB Pathogenesis. Seminars in Immunology, 26(6): 543-51
Forsythe, P. 2013. “Probiotics and Lung Immune Responses”. American Thoracic Society. 11 (1). 33-37.
Kechagia, M., Basoulis, D., Konstntopoulou, S., Dimitriadi D., Gyftopoulou, K., Skarmoutsou, N & Fakiri, E.M. 2013. “Health Benefits of Probiotics : A Review”. Hindawi Publishing Corporation, Article ID 481651.
Mortaz, E., Adcock, I.M., Folkerts, G., Barnes, P.J., Vos, A.P., Garssen, J. 2013. “Probiotics in the Management of Lung Diseases”. Mediators of Inflammation. 1-10.
Nanno, M., Matsumoto, S., and Shida, K. 2014. Chapter 2 : Modulation of Mucosal Immune System by Probiotics : Postulated Mechnisms. In : Kitazawa, H., Villena, J., and Alvarez, S. (Eds.). Probiotics : Immunobiotics and Immunogenics. Boca Raton : Taylor & Francis Group, LLC, pp. 12 – 35.
O’Garra, A., Redford, P.S., McNab, F.W., Bloom, C.I., Wilkinson, R.J., Berry, M.P.R. 2013. “The Immune Response in Tuberculosis”. The Annual Review of Immunology. Vol 31. pp 475-527.
Shetty, P. 2010. “Nutrition, Immunity, and Infection”. Cambridge University Press.
Spinas, E., Saggini, A., Kristas, S.K., Cerulli, G., Caraffa, A., Antinolfi, P., Pantalone, A., Frydas, A., Tei, M., Speziali, A., Saggini, R., Pandolfi, F., Conti, P. 2015. “Crosstalk between Vitamin B and Immunity”. Journal of Biological Regulators and Homeostatic Agents. 29 (2). 283-288.
World Health Organization (WHO), 2018. “Global Report on Tuberculosis report vaccines 2018.
World Health Organization (WHO), 2017. “Global Report on Tuberculosis report 2017
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