Antimicrobial activity and Identification of fungus associated Stylissa flabelliformis sponge collected from Menjangan Island West Bali National Park, Indonesia
The Fungus is a very important microorganism as a producer of bioactive secondary metabolites. Active substances of microbial origin have been sought through the process of screening methods to obtain antimicrobial compounds. The purpose of this study was to isolate fungi associated with sponge taken from Menjangan Island National Park West Bali (Indonesia) and identify fungi that have antimicrobial activity. Isolation of fungus from sponge was carried out by spread plate method using Saboroud Saline Agar medium. Each fungi will be tested to Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Identification of fungi is based on the observation of macroscopic, microscopic and also using 16rRNA/ITS phylogeny tree. The results showed that S. flabelliformis sponge had 10 fungal isolates. Most of them have antimicrobial activity. The name associated with a sponge fungus is These 10 fungus are Aspergillus flavus strain UPMZ02, Aspergillus fumigatus strain CD1621, Trichoderma reesei strain JCM 2267, Aspergillus nomius strain KUB105, Aspergillus sp. strain TLWK-09, Aspergillus flavus strain MC-10-L, Penicillium sp. strain RMA-2, Aspergillus sp. strain TLWK-09, Aspergillus fumigatus and Trichoderma reesei strain TV221
Atashpaza S., Khania S., Barzegaria A., Barara, J., Zununi SVRA, and Omidi Y., 2010, A Robust Universal Method for Extraction of Genomic DNA from Bacterial Species, Microbiology, 79 (4) 538–542.
Bills GF., and Gloer JB., 2016, Biologically Active Secondary Metabolites from the Fungi, Microbiol Spectr, 4(6).
Ebada SS., and Proksch P., 2010, Bioactive Secondary Metabolites from Marine-Derived Fungi in Marine Pharmacognosy Trends and Applications Edited by Se_kwon Kim, WILEY-VCH, Verlag GMBH & Co
Hooper JNA, and van Soest RWM., 2002, Systema Porifera: A Guide to Classification of Sponges, pp: 783-784, volume 1, Kluwer Academic, Plenum Publisher, New York
Hugo WB., Russels AD., 2011, Pharmaceutical Microbiology, 8th Ed., 267-268, Blackwell Scientific Publications, Oxford London Edinburgh, Boston Melbourne.
Indraningrat AAg., Smidt H., and Sipkema D., 2016, Bioprospecting Sponge-Associated Microbes for Antimicrobial Compounds, Mar. Drugs 14(5), 87
Jawetz E., Melnick JL., Adelberg EA., Brooks, GF., Butel JS., Ornston LN., 1995, Jawetz Melnick, Adelberg Mikrobiologi Kedokteran, diterjemahkan oleh Nugroho, E., & Maulany, R.F., 1996, 20th Ed., 208, 211-214, 234-236, Penerbit Buku Kedokteran EGC, Jakarta.
Kim SK., Dewapriya P., and Li YX., 2013, Marine Sponge-Associated Microbes A Source of Biologically Active Metabolites in Marine Pharmacognosy Trends and Applications Edited by Se_Kwon Kim, WILEY-VCH, Verlag GMBH & Co
Koopmans M., Martens D., and Wijffels RH., 2009, Towards Commercial Production of Sponge Medicines, Mar Drugs, 7(4): 787–802
Mehbub MF., Lei J., Franco C., Zhang W., 2014, Marine Sponge Derived Natural Products between 2001 and 2010: Trends and Opportunities for Discovery of Bioactives, Mar. Drugs, 12(8), 4539-4577
Niyomkam P., Kaewbumrung S.,Kaewnpparat S., and Panichayupakaranant P., 2010, Antibacterial activity of Thai herbal extracts on acne involved microorganism, Pharm. Biol., (48) 375-380.
Proksch P, Edrada RA, Ebel R, 2002, Drugs from the seas - current status and microbiological implications, Appl Microbiol Biotech, 59(2-3):125-34
Santosa DA., 2011, Rapid Extraction and Purification of Environmental DNA for Molecular Cloning Applications and Molecular Diversity Studies, Molecular Biotechnology Vol 17, 59-64
Setyowati EP., Jenie UA., Sudarsono, Kardono, LB., 2016, Apoptosis induction of bioactive substance Theonellapeptolide 1d and 1-(tetrahydro-4-hydroxy-5-(hydroxymethyl) furan-2-yl)-5-methyl pyrimidine-2,4(1H,3H)-dione isolated from Kaliapsis sp sponge collection from West Bali National Park Indonesia, J Bio. Scie16 (1-2) 30-36
Setyowati, EP., Sudarsono Wahyuono S., 2004, Uji Sitotoksik dan Uji Antimikroba Senyawa Bioaktif Spons Sylissa flabelliformis, Indonesian J Pharmacy, 15 (2)
Taylor MW., Radax R., Steger D., Wagner M., 2007, Sponge-Associated Microorganism: Evolution, Ecology, and Biotechnological Potential, Microbiol Mol Biol Rev 71(2) 295–347.
Thakur NL., and Müller WEG., 2004, Biotechnological Potential of Marine Sponges, Current Science, 86, 1506-1512
Thomas TRA., Kavlekar DP., and Loka Bharathi PA., 2010, Marine Drugs from Sponge-Microbe Association—A Review, Mar Drugs 8(4) 1417–1468
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