Antimicrobial activity and Identification of fungus associated Stylissa flabelliformis sponge collected from Menjangan Island West Bali National Park, Indonesia

Erna Prawita Setyowati, Silvia Utami Tunjung Pratiwi, Purwantiningsih Purwantiningsih, Oka Samara

Abstract


The Fungus is a very important microorganism as a producer of bioactive secondary metabolites. Active substances of microbial origin have been sought through the process of screening methods to obtain antimicrobial compounds. The purpose of this study was to isolate fungi associated with sponge Stylissa flabelliformis taken from Menjangan Island National Park West Bali (Indonesia) and identify fungi that have antimicrobial activity. Isolation of fungus from sponge was done by spread plate method using SAL medium (Saboroud Saline Agar). Each fungi will be tested with Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Identification of fungi is based on the observation of macroscopic, microscopic and also using 16rRNA /ITS phylogeny tree. The results showed that Stylissa flabelliformis sponge had 10 fungal isolates. Most of them have antimicrobial activity. The name associated with a sponge fungus is Aspergillus flavus, Aspergillus fumigatus, Trichoderma reesei, Aspergillus nomius, Aspergillus sp, Aspergillus flavus, Penicillium sp., Aspergillus sp, Aspergillus fumigatus and Trichoderma sp.

Keywords


Sponge-associated fungi, Stylissa flabelliformis, antimicrobial activity

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DOI: http://dx.doi.org/10.14499/indonesianjpharm29iss2pp%25p

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