Florence Pribadi, Suhartati Suhartati, Achmad Basori


Epidemiology of NSAIDs gastropathy is increasing as increase number of usage. From arthritis to cardiovascular events and cancer prevention, the versatility of NSAIDs is not questioned. However, no dose of NSAIDs is safe. No matter low dose, or single use, NSAIDs will cause gastric damage upto 3-7 days after use. In inflammation and healing process of gastropathy there are various proteins involved, but treatment with COX-2 and SRF are associated with an immediate healing and better quality of gastric mucosa. Cyclea barbata (miers.) has been declared as functional food for preventing and treatment gastropathy yet, its mechanism of actions have not yet clearly discovered. Hence the aim of this study is to analyze the effects of Cyclea barbata (miers.) ethanol extract to COX-2 and SRF in gastric tissue, in time series basis. Laboratory mice was induced with aspirin to produce gastropathy and then treated with Cyclea barbata (miers.) extract for 1, 3, 7, 10 or 14 days. Gastric tissue then harvested and analysed with elisa procedure to determine tissue SRF and COX-2 level. Treatment was proven to increase COX-2 and SRF higher than control group. This concludes one of Cyclea barbata (miers.) mechanism for NSAIDs gastropathy is by increasing tissue COX-2 and SRF. 


Aspirin-induced gastropathy, COX-2, SRF, NSAIDs


Alam, M. N., Faruk, M., Chowdhury, A. I., Ahmed, S. S., Ahmad, M. F., Debnath, M., & Sayeed, M. A. (2016). Comparative Study of Potential Anti-Cancerous and Cardio Protective Activities of Methanolic Leaf Extract of Cycleabarbata and Entadapursaetha. Research Journal of Medicinal Plant, 10(1), 113–119.

Brown, T. J., Hooper, L., Elliott, R. A., Payne, K., Webb, R., Roberts, C., Symmons, D. (2006). A comparison of the cost-effectiveness of five strategies for the prevention of non-steroidal anti-inflammatory drug-induced gastrointestinal toxicity: a systematic review with economic modelling. Health Technology Assessment, 10(38), 202.

Chai, J., Baatar, D., Moon, W., & Tarnawski, A. A. S. (2004). Expression of serum response factor in normal rat gastric mucosa. Gastroenterology, 126, 1809–1818.

Chen, P., Qin, L., Li, G., Tellides, G., & Simons, M. (2016). Fibroblast growth factor ( FGF ) signaling regulates transforming growth factor beta ( TGF β ) - dependent smooth muscle cell phenotype modulation. Nature Publishing Group, (August), 1–11.

Farzaei, M. H., Abdollahi, M., & Rahimi, R. (2015). Role of dietary polyphenols in the management of peptic ulcer. World Journal Gastroenterology, 21(21), 6499–6517.

Lucena Périco, L., Cristina Heredia-Vieira, S., Pereira Beserra, F., de Cássia dos Santos, R., Barczyszyn Weiss, M., Aparecida Resende, F., Akiko Hiruma-Lima, C. (2015). Does the gastroprotective action of a medicinal plant ensure healing effects? An integrative study of the biological effects of Serjania marginata Casar. (Sapindaceae) in rats. Journal of Ethnopharmacology, 172, 312–324.

Melcarne, L., García-Iglesias, P., & Calvet, X. (2016). Management of NSAID-associated peptic ulcer disease. Expert Review of Gastroenterology & Hepatology, 10(6), 723–733.

Permanasari, D. A. (2015). Aktivitas ekstrak etanol daun cincau hijau ( Cyclea barbata Miers ) sebagai penghambat pembentukan biofilm bakteri Salmonella typhi. University of Jember.

Ro, S., & Words, K. (2016). Multi-phenotypic Role of Serum Response Factor in the Gastrointestinal System, 22(2), 193–200.

S. Tarnawski, A., & Ahluwalia, A. (2012). Molecular mechanisms of epithelial regeneration and neovascularization during healing of gastric and esophageal ulcers. Current Medicinal Chemistry, 19(1), 16–27.

Samara De Lira Mota, K., Nunes Dias, G. E., Ferreira Pinto, M. E., Luiz-Ferreira, Â., Regina, A., Souza-Brito, M., Batista, L. M. (2009). Flavonoids with Gastroprotective Activity. Molecules, 14, 979–1012.

Shodiq Ali Muhammad. (2012). uji aktivitas antioksidan ekstrak dan fraksi daun cincau hijau rambat (Cyclea barbata Miers.) dan identifikasi golongan senyawa dari fraksi yang paling aktif. University of Indonesia.

Simon, J. P., Evan Prince, S., & Prince, S. E. (2017). Natural remedies for non-steroidal anti-inflammatory drug-induced toxicity. Journal of Applied Toxicology, 37(August 2016), 71–83.

Soylu, A., Dolapcioglu, C., Dolay, K., Ciltas, A., Yasar, N., Kalayci, M., Sever, N. (2008). Endoscopic and histopathological evaluation of acute gastric injury in high-dose acetaminophen and nonsteroidal anti-inflammatory drug ingestion with suicidal intent. World Journal Gastroenterology, 14(43), 6704–6710.

Tarnawski, A. S., Ahluwalia, A., & Jones, M. K. (2012). The mechanisms of gastric mucosal injury: Focus on microvascular endothelium as a key target. Current Medicinal Chemistry, 19(1), 4–15.

Taylor, A., Tang, W., Bruscia, E. M., Zhang, P.-X., Lin, A., Gaines, P., Halene, S. (2014). SRF is required for neutrophil migration in response to inflammation. Blood, 123(19), 3027–3036.

Trimurtini, I. (2007). Efek anti ulkus ekstrak air daun cincau hijau (Cyclea barbata miers) terhadap mukosa dan kadar mukus gaster tikus jantan galur wistar yang diinduksi aspirin. Medika Kartika, 4(1), 1–15.

Verpoorte R, Van Beek TA, Siwon H, B. svendsen. (1982). Studies on Indonesian medicinal plants VIII . Screening of some Indonesian Menispermaceae for alkaloids ; the isolation of magnoflorine from pycnarrhena novoguinensis and Cyclea barbata. Pharmaceutisch Weekblad Scientific Edition, 4, 87–88.

Wallace, J. L., & L., J. (2006). COX-2: a pivotal enzyme in mucosal protection and resolution of inflammation. The Scientific World Journal, 6, 577–88.



  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:


analytics View My Stats