INHIBITORY ACTIVITY OF ENDOPHYTIC FUNGI ISOLATED FROM SUKABUMI TURMERIC PLANT (Curcuma longa L.) AGAINST MCF-7 CELL LINE
Kunyit or turmeric plant (Curcuma longa L.) is a native Southeast Asia plant that has been widely used as herbal medicine. In our previous research we isolated and screened 44 of endophytic fungi of turmeric plant from Sukabumi and Cibinong to determine their antioxidant activity. There were 4 samples isolated from Sukabumi with antioxidant activity for more than 70% at concentration of 100ppm, K.Cl.Sb.R9 (93%), K.Cl.Sb.A11 (81%), K.Cl.Sb.B1 (79%) and K.Cl.Sb.R11 (71%). This research aimed to determine inhibitory activity of the endophytic fungi against estrogen positive MCF-7 breast cancer cell line. To obtain the filtrate, the broth cultures were filtered and the extracellular fraction was extracted with ethyl acetate. The inhibitory activity was determined by using MTT assay. The result showed that the ability of endophytic fungi to inhibit MCF-7 growth was dose dependently. IC50 of endophytic fungi K.Cl.Sb.R9 was 579±38 ppm, K.Cl.Sb.R11 was 542±21ppm, K.Cl.Sb.B1 was 446±15ppm and K.Cl.Sb.A11 was 520±28ppm. Fluorescent double staining based method using calcein AM and ethidium bromide was performed to confirm the inhibitory activity. At 500ppm of filtrate concentration with 24h of cell treatment, treated cell lines showed fewer viable cells compared to untreated cell lines. The four isolates of endophytic fungi were able to inhibit proliferation of human breast cancer MCF-7 cell line.
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Alvin, A., Miller, K.I., Neilan, B.A. (2014), Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds, Microbiological Research, 169(7-8): 483-495.
Arau´jo, W. L., Marcon, J., Maccheroni Jr, W.,van Elsas, J. D. & Azevedo, J. L. (2002). Diversity of Endophytic Bacterial Populations and Their Interaction with Xylella fastidiosa in Citrus Plants. Applied and Environmental Microbiology. 68(10) : 4906-4914.
Ashraf, A., Javaid, A. (2005), Fungi associated with rhizome of turmeric (Curcuma longa L.) in Pakistan, Mycopath, 3(1/2): 69-71.
Balakumaran, M.D., Ramachandran, R., Kalaichelvan, P.T. (2015) : Exploitation of Endophytic Fungus, Guignardia Mangiferae for extracellular Synthesis of Silver Nanoparticles and Their in Vitro Biological Activities, Microbial Research, 178, 9-17.
Bustanussalam, Rahman, F., Septiana, E., Widowati, T., Lekatompessy, S., Sukiman, H., Simanjuntak P., (2015). Screening of Endophytic Fungi from Turmeric Plant of Sukabumi and Cibinong with Potency as Antioxidant Compound Producer, Pakistan J Biologycal sciences, 18(1): 42-45.
Bernhard, H., Jäger-Arand, E., Bernhard, G., Heike, M., Klein, O., Riemann, J.F., Meyer zum Büschenfelde, K.H., Dippold, W. And A. Knuth. 1995. Treatment of advanced pancreatic cancer with 5-fluorouracil, folinic acid and interferon alpha-2A: results of a phase II trial, British J. Cancer. 71(1): 102–105.
Carvalho, P., Amaral, P., Ruiz, A., Alenca, Sr., Pfenning, L., Carvalho, J., Rosalen, P., Ikegaki, M. (2012). Paraconiothyrium sp. P83F4/1: antioxidant and antiproliferative activities of endophytic fungus associated with Rheedia brasiliensis plant, International Journal of Biotechnology for Wellness Industries, 1, 172-176
Chapla, V. M., Zeraik, M. L., Leptokarydis, I. H., Silva, G. H., Bolzani, V. S., Young, M. C. M., Pfenning, L. H., Araujo, A. R. (2014). Antifungal Compounds Produced by Colletotrichum gloeosporioides, an Endophytic Fungus from Michelia champaca. Molecules. 19: 19243-19252.
Dompeipen, E.J., Simanjuntak, P. (2015), Aktivitas antidiabetes dan antioksidan kapang endofit dari tanaman mahoni, Biopropal industri, 6(1): 7-17.
Gangadevi, V., Muthumary, J. (2008), Isolation of Colletotrichum gloeosporioides, a novel endophytic taxol-producing fungus from the leaves of a medicinal plant, Justicia gendarussa. Mycologia Balcanica, 5: 1-4.
Hulikere, M.M., Joshi, C.G., Nivvya, T., Ananda, D., Raju, N.G. (2016) : Antiangiogenic and Antioxidant Activity of Endophytic Fungus Isolated from Seaweed (Sargassum wightii), Asian J Biochemistry, 11(4): 168-176.
Krup, V., Prakash, H., Harini, A., (2013). Pharmacological Activities of Turmeric (Curcuma longa linn): A Review, Homeopathy & Ayurvedic Medicine, 2(4) :1-5
Ikpeama, Ahamefula, Onwuka, GI. Nwankwo, C.(2014). Nutritional Composition of Tumeric (Curcuma longa) and its Antimicrobial Properties, International J Scien Eng Res, 5(10): 1085-1089.
Lee, K.H., Lee, J.S., Suh, C., Lee, Y.S., Min, Y.I., Ahn, S.H., Park, K.C., Kim, S.K. And Kim, S.H. 1992. Combination of 5-fluorouracil and recombinant interferon alpha-2B in advanced gastric cancer. A phase I study, Am J. Clin Onc. 15(2) : 141-145.
Li, S., Yuan, W., Deng, G., Wang, P., Yang, P. (2011). Chemical composition and product quality control of turmeric (Curcuma longa L.), Pharmaceutical Crops, 2011, 2: 28-54.
Lippmann, M.E., Bolan, G. (1975). Oestrogen-responsive human breast cancer in long-term tissue culture. Nature, 256,593-595
Lin, T.B., Chung, D. L., Chang, J.R. (1999), Ethyl Acetate Production from Water-Containing Ethanol Catalyzed by Supported Pd Catalysts: Advantages and Disadvantages of Hydrophobic Supports, Industrial Engineering Chemistry Reports, 38(4): 1271-1276.
Lindner, D.J., Borden, E.C. And Kalvakolanu, D.V. 1997. Synergistic antitumor effects of a combinantion of interferons and retinoic acid on human tumor cells in vitro and in vivo, clinical cancer research. 3: 931-937.
Papadopoulos, N.G., Dedoussis, G.V., Spanakos, G., Gritzapis, A.D., Baxevanis, C.N. And Papamichail, M. 1994. An improved fluorescence assay for the determintion of lymphocyte-mediated cytotoxicity using flocitomety, J. Immunology Methods.
Maehara, S., Ikeda, M., Haraguchi, H., Kitamura, C., Nagoe, T., Ohashi, K., Shibuya, H. (2011), Microbial conversion of curcumin into colorless hydroderivatives by the endophytic fungus Diaporthe sp. associated with Curcuma longa, Chem Pharm Bull, 59(8) : 1042-1044.
Ningrum, R.A., Wisnuwardhani, P.H., Santoso, A., Herawati, N. (2015). Antiproliferative Activity of Recombinant Human Interferon alpha2b on MCF-7 cell line, Indonesian J. Pharm, 26(2): 86-93.
Ningrum, R.A., Wisnuwardhani, P.H., Santoso, A., Herawati, N. (2015), Antiproliferative Activity of Recombinant Human Interferon alpha2b on MCF-7 cell line, Indonesian JPharm, 26(2): 86-93.
Sadananda, T.S, Govindappa, M., Ramachandra, Y.L., Chandrappa, C.P., Umashankar, T.(2016), In Vitro Apoptotic Activity of Endophytic Fungal Lectin Isolated from Endophyte, Aspergillus flavus of Viscum album on Human Breast Adenocarcinoma Cell Line (MCF-7), Metabolomics, 6(1):1-7.
Septisetyani, E.P., Ningrum, R.A., Romadhani, Y., Wisnuwardhani, P.H., Santoso, A. (2014), Optimization of sodium dodecyl sulphate as a formazan solvent and comparison of MTT assay with WST-1 assay in MCF-7 cells, Indonesian J Phar, 25 (4) : 245-254.
Singh, D., Rathod, V., Ninganagouda, S., Herimath, J., Kulkarni, P. (2013), Biosynthesis of silver nanoparticle by endophytic fungi Pencillium sp. isolated from Curcuma longa (turmeric) and its antibacterial activity against pathogenic gram negative bacteria, J. Pharm. Res, 7(5) : 448-453.
Suryanarayanan, T.S., Thirunavukkarasu, N., Govindarajulu, M.B., Sasse, F., Jansen, R., Murali, T.S. (2009), Fungal endophytes and bioprospecting, Fungal biology review, 23: 9-19.
Taylor, C.W., Modiano, M.R., Woodson, M.E., Marcus, S.G., Alberts, D.S. And Hersh, E.M. 1992. A phase I trial of fluorouracil, leucovorin, and recombinant interferon alpha-2b in patients with advanced malignancy, Seminanr in Oncology. 19 (2 Suppl 3):185-190.
Tianpanich, K., Prachya, S., Wiyakrutta, S., Mahidol, C., Ruchirawat, S., Kittakoop, P. (2011). Radical Scavenging and Antioxidant Activities of Isocoumarins and a Phthalide from the Endophytic Fungus Colletotrichum sp. J Nat Prod. 74, 79-81.
Vasundhara, M., Baranwal, M., Kumar, A. (2016). Fusarium tricinctum, An Endophytic Fungus Exhibits Cell Growth Inhibition and Antioxidant Activity, Ind J microbiology, 56(4):433-438.
Vennila, R., Kamalraj, S., Muthumary, J. (2012), In vitro Studies on anticancer activity of fungal taxol against human breast cancer cell line MCF-7 cells, Asian Pacific J.Trop Biomed, S1159-S1161
Widowati, T., Bustanussalam, Sukiman, H., Simanjuntak. P. (2016). Isolasi dan karakterisasi kapang endofit dari tanaman kunyit sebagai penghasil antioksidan, Biopropal industri, 7(1):9-16.
Zakiyah, A., Radiastuti, N., Sumarlin, L.O. (2013). Aktivitas antibakteri kapang endofit dari tanaman kina, Al kauniyah Jurnal Biologi, 8(2) : 51-58.
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