Evaluation of In vitro and In vivo Antioxidant potential of Morinda reticulata Gamble Tubers in Wistar Albino Rats Subjected to CCl4 and Paracetamol induced Hepatotoxicity

Raju Asirvatham, Joshila Jose Usha

Abstract


The aim of present study is to explore the antioxidant potential of ethanol and aqueous extracts of Morinda reticulata Gamble by in vitro and in vivo methods.  In vitro antioxidant activity of Benzene, Chloroform, Ethanol and aqueous extracts of  M. reticulata was studied by DPPH, Metal chelating, Super oxide free radical scavenging assay, Hydroxyl radical scavenging assay and Reducing power assay where ascorbic acid was used as a standard antioxidant. Oxidative stress in Wistar rats was induced by two different models using administration of CCl4 (1.5 ml/kg, p.o) and paracetamol (2g/kg) on seventh day of study. Ethanol and aqueous extracts were given twice daily for one week. Silymarin (25 mg/kg, p.o) was given as a standard drug. In in vitro, IC50 values were least with ethanol and aqueous extracts when compared with other extracts. Ethanol extracts exhibited similar free radical scavenging effect as that of standard ascorbic acid.  In vivo antioxidant activity was assessed by the measurement of Melondyaldehyde (MDA), Reduced Glutathione (GSH), Super oxide dismutase (SOD) and Total protein levels were estimated from the liver tissue homogenate. The level of MDA (3.27±2.18) was significantly (p˂0.001) increased whereas decreased level of total protein, GSH and SOD were found in CCl4 and paracetamol control group. Seven days extracts treatments restored these altered parameters in to normal where ethanol extract treatment group showed significant (p˂0.001) result than aqueous extract. HPTLC study showed that presence of six phytoconstituents with corresponding Rf value. The study report concluded that M.reticulata has very good antioxidant effect against CCl4 and paracetamol induced liver damage with oxidative stress. Probable mechanism behind this protection against oxidative damage produced by CCl4 and paracetamol is its phytoconstiuents.


Keywords


M.reticulata; in vitro and in vivo antioxidant; DPPH; Sylimarin; Melondyaldehyde (MDA); Reduced Glutathione (GSH); Super oxide dismutase (SOD)

References


Bhaskar R., Rajeswary V., Satheesh Kumar., 2006. In vitro antioxidant studies on leaves of Annona species. Indian J of Experimental Biology. 45:480-485.

Carmel Punitha S., Rajasekaran M., 201. Antioxidant mediated defense role of Wedelia calendulacea herbal extract against Ccl4 induced toxic hepatitis. Int J Pharm Sci. 1 (9): 111-115.

Deepak K., Dash Veerendra C., Yeligar Siva S., Nayak TirthaGhosh D., Rajalingam., PinakiSengupta., Bhim C., Maiti Tapan KM., 2007. Evaluation of hepatoprotective and antioxidant activity of Lchnocarpus frutescens (Linn.) R.Br. on Paracetamol induced hepatotoxicity in rats. Tropical J of Pharmaceutical Research. 6 (3): 755-765.

Donatus IA., Sardjoko., Vermeulen NP., 1990. Cytotoxic and cytoprotective activities of curcumin. Effects on paracetamol induced cytotoxicity, lipid peroxidation and glutathione depletion in rat hepatocytes. Biochem Pharmacol. 39:1869–1875.

Ellman LG., Courtney KD., Andres JV., Featherstone RM., 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 7 : 88–95.

Farida Yesmin., Zaida Rahman., Jesmin Fouzia D., Asadul Mazid H., NorIza AR., Ahmed G., Alattraqchi AA., Rabeya Yousuf., Abdus Salam., Mainul H., 2013. Hepatoprotective role of the aqueous and n-hexane extracts of Nigella sativa Linn. in experimental liver damage in rats. Asian J Pharm Clin Res. 6(3):2013, 205-209.

Hassan BAR., 2012. HPLC uses and importance in the Pharmaceutical Analysis and industrial field. Pharmaceut Anal Acta. 3: e133.

Ijinu TP., Anish N., Shyju H., 2011. Home Gardens for nutrition and primary health security of rural poor of south Kerala. Indian J Traditional Knowledge. 10(3): 413-428.

Jayanthi P., Lalitha P., 2014. Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int J Pharm Pharm Sc. 3 (3): 126-128.

Jayasri P., Elumalai A., Narendra Naik D., Kalugonda MK., 2011. In-vitro antioxidant activity of Sidaspinosa Linn. J Nat Prod Plant Resour. 1 (4):35-39.

Kokate CK., 2007. Text book of Pharmacognosy, Nirali Publications, New Delhi. 1-73.

Kumaravel RS., Maleeka Begum SF., .Parvathib H., Senthil Kumar CM., 2013. Phytochemical screening and in vitro antioxidant activity of ethyl acetate leaf extracts of Pterocarpus marsupium Roxb (Fabaceae). Int J Curr Sci. 9: E 46-55.

Lowry OH., Rosebrough NJ., Farr AL., Randall RJ., 1951. Protein measurement with the Folin phenol reagent. J Biol Chem. 193 (1): 265–75.

Mahmood Reza M., Soheila Moein., Saeid Ahmadizadeh., 2008. Radical scavenging and reducing power of Salvia mirzayanii subfractions. Molecules 13: 2804-2813.

Marijana Zovko Koncic., Monika Barbaric., Ivana Perkovic., Branka Zorc., 2011. Antiradical, chelating and antioxidant activities of Hydroxamic acids and Hydroxyureas. Molecules.16: 6232-6242.

Mohammad N., Qureshi Bhanudansh S., Kuchekar Nadeem A., Logade Majid AH., 2010. In vitro antioxidant and in vivo hepatoprotective activity of Leuca sciliata leaves. Rec. Nat. Prod. 4(2):124-130.

Olivier P., Roger VF., Petur W., Dalsgaard MH., 2007. Identification of TLC markers and quantification by HPLC-MS of various constituents in Noni fruit powder and commercial Noni derived products. J Agric Food Chem. 55: 7489-7494.

Organisation for Economic Cooperation and Development (OECD); Guidelines for Testing of Chemicals 423, Acute oral toxicity acute toxic classmethod,http://www.iccvam.niehs.nih.gov/SuppDocs/OECD/OCDE_GL423.

Oyedemi SO., Bradley G., .Afolayan A., 2010. In vitro and in vivo antioxidant activities of aqueous extract of Strychno shenningsii Gilg. African J of Pharmacy and Pharmacology. 4(2): 70-78.

Periasamy M., Pavankumar K., Venkata Gangadhar V., Jeeva T., Anandhan R., 2012. Hepatoprotective and Antioxidant activity of Euphorbia ligularia against Carbon Tetrachloride Induced Hepatotoxicity in Wistar rats. IJRPBS. 3 (1): 2229-3701.

Ragıp Balahoroğlu., Haluk Dülger., Hanefi Özbek., İrfan Bayram., Mehmet Ramazan Şekeroğlu., 2008. Protective effects of antioxidants on the experimental liver and kidney toxicity in mice. Eur J Gen Med. 5(3):157-164.

Rahul Raveendran Nair., Murugan Kavitha., Sethuraman Thilaga., Doss Ganesh., 2012. Conservation and in vitro multiplication of highly endangered Indian traditional medicinal plant (Morinda reticulate Gamble) through nodal explants. Plant Knowledge J. 1(2):46-51.

Raju A., Christina AJM., 2013b. Drosera burmannii Vahl: Antioxidant potential in Dalton’s Ascites Lymphoma (DAL) bearing mice. Journal of Medicinal Plants Studies. 1(4):151-159.

Raju A., Christina AJM., Anita Murali., 2013a. In Vitro Antioxidant and anticancer activity studies on Drosera indica L. (Droseraceae). Adv Pharm Bull. 3(1): 115-120.

Satheesh Kumar D., Kottai Muthu A., Anton Smith A., Manavalan R., 2010. In vitro antioxidant activity of various extracts of whole plant of Mucuna pruriens (Linn). Int.J PharmTech Res. 2(3): 2063-2070.

Thangaraj Francis Xavier., Moorthy Kannan., Leyone Lija., Anthonysamy Auxillia., Antony Kanthi Freeda Rose., Subburaman Senthilkumar., 2014. Ethnobotanical study of Kani tribes in Thodu hills of Kerala, South India. Journal of Ethnopharmacology. 152:78–90.




DOI: http://dx.doi.org/10.14499/indonesianjpharm28iss3pp%25p

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