CYTOTOXIC AND ANTI-VIRAL ACTIVITY OF Acacia catechu ON HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS

Amit Gupta, Sushama R. Chaphalkar

Abstract


As per the literature, medicinal plants showed enormous candidates who is responsible or showing anti-microbial, anti-cancer, anti-diabetic, anti-inflammatory agents etc. Most of the research group focused only on those primary and secondary metabolites extracted from different medicinal plants and showed its antimicrobial (anti-viral) activity against dreadful pathogens. The objective of our current study is to evaluate the cytotoxic and anti-viral effect of aqueous leaves extract of Acacia catechu against new castle disease Virus (NDV) on human peripheral blood mononuclear cells (PBMC). For these studies, variable doses of aqueous leaves extract of Acacia catechu (0.5–30mg/mL, 50µL; dissolved in phosphate buffered saline, PBS) and examined its proliferation assay containing NDV and also determined CD14 monocyte surface marker in presence or absence of NDV using flow cytometry. The results showed that aqueous leaves extract of Acacia catechu inhibited NDV proliferation and also decline in CD14 monocyte surface marker with or without NDV at higher doses. Overall, aqueous extract of Acacia catechu at higher doses showed cytotoxic as well as antiviral effect and might be used for this purpose.


Keywords


Acacia catechu; NDV; aqueous extract

Full Text:

PDF (111-116)

References


Gupta A, Khamkar PR and Chaphalkar SR. Review on medicinal plants to target and inhibit the epidermal growth factor receptor signaling in cancer and tissue repair therapy. International Journal of Advances in Pharmacy, Biology and Chemistry. 2014; 3(1): 210- 213.

Maciel MAM, Pinto AC, Veiga Jr VF, Grynberg NF, Echevarria A. Medicinal plants: the need for multidisciplinary scientific studies. Quim Nova 2002; 25(3):429-438.

Maithani A, Versha P, Geeta P, Kumar D, Dhulia I. Pharmacognostical standardization of leaves of Azadirachta Indica- An effective panacea for all disease. Int J Res Pharm Sci 2011; 2(4): 563 – 568.

Gupta A, Khamkar PR and Chaphalkar SR. Anti-inflammatory activity of aqueous extract of Mimusops elengi on human whole blood and peripheral blood mononuclear cells. International Journal of Current trends in Pharmaceutical research 2014; 2: 638 – 645.

Patil SM and Saini R. Antimicrobial activity of flower extract of Calotropis gigantea. Int. J Pharm Phytopharmacological Research 2014; 1 (4): 142-145.

Gupta A, Jagtap RB and Chaphalkar SR. Anti-viral activity of Azadirachta indica leaves against Newcastle disease virus: A study by in vitro and in vivo immunological approach. International Journal of Current trends in Pharmaceutical research 2014; 2: 494 - 501.

Lakshmi T and Rajendran R. Estimation of biofavonoid quercetin in Acacia catechu ethanolic leaf extract by HPLC method. International Journal of Pharma and Biosciences 2012; 3 (1): 171 – 178.

Ray D, Sharatchandra K and Thokchom I. Antipyretic, antidiarrhoeal, hypoglycaemic and hepatoprotective activities of ethyl acetate extract of Acacia catechu wild. In albino rats. Ind J of pharmacology 2006; 38(6):408-13

Guleria S, Tiku A, Singh G, Vyas D and Bhardwaj A. Antioxidant Activity and Protective Effect Against Plasmid DNA Strand Scission of Leaf, Bark, and Heartwood Extracts from Acacia catechu. J of Food Science 2011; 76(7): 959-64.

Gupta A and Chaphalkar SR. Immunopharmacological screening of aqueous root extract of Santalum album. Journal of Herb Med Pharmacology 2016; 5 (1):07 - 11.

Gupta A and Chaphalkar SR. Potential immunosuppressive and anti-inflammatory activity of aqueous extract of Mangifera indica. Advanced herbal medicine 2015; 1(4): 47 – 54.

Gupta A and Chaphalkar SR. Inhibitory potential of aqueous leaves extract of Messua ferrea and Mimusops elengi on antigen specific immune response using human whole blood. Asian Journal of medical and Pharmaceutical researches 2015, 5 (3): 22 – 26.

Haider S, Nazreen S, Alam MM, Gupta A, Hamid H and Alam MS. Anti-inflammatory and anti-nociceptive activities of ethanolic extract and its various fractions from Adiantum capillus veneris Linn. Journal of Ethnopharmacology 2011; 138 (3): 741-747.

Gupta A and Chaphalkar SR. Immunorestorative and anti-inflammatory activity of leaf aqueous extract of Calotropis gigantean using flow cytometry. International Journal of drug discovery and herbal research 2014; 4(4): 761 – 765.

Gupta A and Chaphalkar SR. Immunopharmacological activity of Zingiber officinale on human peripheral blood mononuclear cells. Asian Journal of Medical and Pharmaceutical researches 2015; 5(2): 13 – 17.

Gupta A, Khajuria A, Singh J, Bedi KL, Satti NK, Dutt P, Suri KA, Suri OP and Qazi GN. Immunomodulatory activity of biopolymeric fraction RLJ-NE-205 from Picrorhiza kurroa. International Immunopharmacology 2006; 6 (10): 1543 - 1549.




DOI: http://dx.doi.org/10.14499/indonesianjpharm27iss2pp111

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

web
analytics View My Stats