ANTIOXIDANT, ANTIBACTERIAL POTENTIAL AND HPLC ANALYSIS OF Dioscorea alata BULB

Md. Anisuzzman - -, Md. Nazmul Hasan Zilani, Sharmin Sultana Khushi, Md. Asaduzzman - --, Md. Golam Hossain -

Abstract


  

The purpose of the present study was to evaluate antioxidant, antibacterial potential and HPLC analysis of methanol extract of Dioscorea alata bulb. Antioxidant activity investigated by DPPH free radical scavenging assay, the IC50 value of plant extract was24.99µg/mL. Significant absorption of extract (1.317 of 1mg/mL) showed the ferric reducing activity. HPLC analysis indicated the presence of myricetin (471.53mg/100 g of dry extract) in the highest concentration. Extract showed significant zone of inhibition in antibacterial activity test. The presence of myricetin along with other detected polyphenols might contribute to antioxidant and antibacterial activities.

 

Key words: Total Phenolic content, Myricetin, Scavenging activity, Disc diffusion

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References


Arnous A., Makris DP., Kefalas P., 2001. Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. J. Agric. Food. Chem., 49:5736-5742.

Cheng WY., Kuo YH., Huang CJ., 2007. Isolation and identification of novel estrogenic compounds in yam tuber (Dioscorea alata Cv. Tainung No. 2). J. Agric. Food. Chem., 55(18):7350-58.

Coursey DG., 1967. Yams: an account of the nature, origins, cultivation, and utilization of the useful members of Dioscoraceae. London: Longmans, Green and Co. Ltd. pp. 56-65

Emira N., Mejdi S., Inès N., Eulogio V., Mahjoub A., Abdulbasit A., 2014. Comparative study on the antifungal and antioxidant properties of natural and colored Juglans regia L. barks: A high activity against vaginal Candida strains. Life. Sci. J., 11(8): 327-35.

Ghani A., 2003. Medicinal Plants of Bangladesh. 2nd edition. Dhaka: Bangladesh, The Asiatic Society of Bangladesh. pp.13-18.

Ghani A., 2005. Practical Phytochemistry. Parash Publishers, Dhaka, Bangladesh. pp 8-20.

Gramshaw JW., Osinowo FAO., 1982. Volatile components of cooked tubers of the water yam (Dioscorea alata). J. Sci. Food. Agri., 33(1): 71–80.

Hemayet H., Ismet AJ., Sariful IH., Jamil AS., Shubhra KD., Arpona H., Arif A.. 2013. Anti-inflammatory and antioxidant activities of ethanolic leaf extract of Brownlowia tersa (L.) Kosterm. Orient. Pharm. Exp. Med., DOI 10.1007/s13596-013-0109-3.

Islam MK., Nripendra NB., Sanjib S., Hemayet H., Ismet AJ., Tanzir AK., Khalijah A., Jamil AS., 2014. Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds. Sci.World J., Article ID 869537, 7 pages.

Ismet AJ., Proity NA., Nasima K., Tanzir AK., Mohammad MR., Arpona H., Hemayet H., 2014. Comparative Study of anti-nociceptive activity and phenolic content of the ethanol extracts of Piper nigrum and Piper longum fruits. Int. J. Pharm. Sci. Rev. Res., 27(1): 47-52.

Kochhar SL., 1981. Tropical crops: A textbook of economy botany. London: Macmillan Pub Ltd. pp.125-132.

Ksouri R., Fallah H., Megdchi W., Trabelsi N., Mhamdi B., Chaieb K., Bakrouf A., Magny C., Abdelly C., 2009. An antioxidant and antimicrobial activity of edible medicinal halophyte Tamarix gallica L. and related polyphenolic constitutes. Food. Chem. Toxicol., 47: 2083-2091.

Lula N., Aman W., Nuri A., Eko HP., Takahiro N., Koji I., 2015. Chemical Characterization of Flour Fractions from Five Yam (Dioscorea alata) Cultivars in Indonesia. J. Eng. Technol. Sci., 47(1): 92-103.

Miliauskas G., Venskutonis PR., VanBeek JA., 2004. Screening of free radical scavenging activity of some medicinal and aromatic plants. Food. Chem., 85: 231-237.

Monika M., Nomita G., Palak P., Varsha M., Manisha K., 2014. Phytochemical evaluation and pharmacological activity of syzygium aromaticum: a comprehensive review. Int. J. Pharm. Pharm. Sci., 6(8): 67-72.

Moriya C., Hosoya T., Agawa S., Sugiyama Y., Kozone I., Shin-Ya K., Terahara N., Kumazawa S., 2015. New acylated anthocyanins from purple yam and their antioxidant activity. Biosci. Biotechnol. Biochem.,79(9):1484-1492.

Osagie AU., 1992. The yam tuber in storage. Nigeria: University of Benin, Post Harvest Research Unit.

Pervin R., Farah S, Billah MM.,.Didarul IKM., Elmezughi J., Shilpi JA., 2013. Antibacterial and cytotoxic activity of Meyna spinosa ROXB. stem. Turk. J. Pharm. Sci., 9(2): 225-230.

Rice-Evans C., Sampson J., Bramley PM., Holloway DE.. 1997. Why do we expect carotenoids to be antioxidants in vivo? Free Radical Res., 26: 381-398.

Saha S., Islam MK., Anisuzzman M., Hasan MM., Hossain F., Talukder C., 2012. Evaluation of antioxidant, analgesic and antidiarrhoeal activity of Phoenix paludosa Roxb. leaves. Int. J. Basic. Med. Sci. Pharm., 2: 46-52.

Saha S., Shilpi JA., Mondal H., Gofur R., Billah M., Nahar L., Satyajit DS., 2013. Bioactivity studies on Musa seminifera Lour. Phcog. Mag., 9(36): 315-322.




DOI: http://dx.doi.org/10.14499/indonesianjpharm27iss1pp9

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